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PMID: 6366514 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of the centromere from chromosome V (CEN5) of Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 4 ·No. 1 ·1984-01-00 ·Pages 86-91

Maine GT, Surosky RT, Tye BK

Abstract

We have cloned a functional centromeric DNA sequence from Saccharomyces cerevisiae. Using the 2 mu chromosome-loss mapping technique and meiotic tetrad analysis, we have identified this DNA sequence as the centromere of chromosome V (CEN5). The CEN5 sequence has been localized on an 1,100-base-pair BamHI-BglII restriction fragment. Plasmids containing CEN5 and an autonomously replicating sequence are mitotically stable in S. cerevisiae and segregate in a Mendelian fashion during meiosis.

MeSH Terms
Centromere Chromosome Mapping Chromosomes Cloning, Molecular DNA, Fungal/genetics,isolation & purification Genes, Fungal Meiosis Mitosis Plasmids Saccharomyces cerevisiae/genetics
Chemicals
DNA, Fungal
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Maine G T
Surosky R T
Tye B K
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25 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.1.86-91.1984
Published
1984-01-00
Pages
86-91
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368661
Subset
IM
Grants
NIAID NIH HHS · AI14980 · United States
NIGMS NIH HHS · GM07273 · United States
NIGMS NIH HHS · GM26941 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.1.86-91.1984 PMC Full Text View journal details
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