Fluorescent labelling of tRNA and oligodeoxynucleotides using T4 RNA ligase.

Nucleic acids research ·Vol. 12 ·No. 4 ·1984-02-24 ·Pages 1791-810

Cosstick R, McLaughlin LW, Eckstein F

Abstract

3'-O-(5'-phosphoryldeoxycytidyl) phosphorothioate and fluorescent 3'-O-(5'-phosphoryldeoxycytidyl) S-bimane phosphorothioate can be ligated to tRNA by T4 RNA ligase. They are also efficient donors for the enzymatic ligation to oligodeoxynucleotides bearing a 3'-cytidine terminus. Cytidine 3',5'-bisphosphate is also a substrate for the ligation reaction with DNA restriction fragments with a 3'-terminate cytidylic acid residue. Oligo- and polynucleotides with a 3'-phosphorothioate group react readily with electrophiles as exemplified by the reaction with monobromobimane.

MeSH Terms

Bridged Bicyclo Compounds/chemical synthesis Bridged-Ring Compounds/chemical synthesis Chromatography, High Pressure Liquid Deoxycytosine Nucleotides/chemical synthesis Escherichia coli/enzymology Fluorescent Dyes/chemical synthesis Oligodeoxyribonucleotides/metabolism Oligonucleotides/metabolism Polynucleotide Ligases/metabolism RNA Ligase (ATP)/metabolism RNA, Transfer/genetics Structure-Activity Relationship T-Phages/enzymology Thionucleotides

Chemicals

3'-O-(5'-phosphoryldeoxycytidyl) S-bimane phosphorothioate 3'-O-(5'-phosphoryldeoxycytidyl)phosphorothioate Bridged Bicyclo Compounds Bridged-Ring Compounds Deoxycytosine Nucleotides Fluorescent Dyes Oligodeoxyribonucleotides Oligonucleotides Thionucleotides RNA, Transfer Polynucleotide Ligases RNA Ligase (ATP)

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Cosstick R

McLaughlin L W

Eckstein F

References (25)

25 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/12.4.1791

Published

1984-02-24

Pages

1791-810

Language

English

Region

England

NLM ID

0411011

PMCID

PMC318621

Subset

External Links

PubMed source DOI: 10.1093/nar/12.4.1791 PMC Full Text View journal details

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Fluorescent labelling of tRNA and oligodeoxynucleotides using T4 RNA ligase.

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