Two fertility inhibition (Fi+) functions which reduce R388 (Inc W) transfer were detected on RP1 (Inc P). Neither function affected R388 -mediated surface exclusion but they could be distinguished by their effect on pilus production. One of the functions was located in the 6.5-kb Pst1 -C region of RP1, part or all of which also occurs on six Fi+ but not two Fi- Inc P plasmids studied.
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