Plasmid-mediated complementation of a delta-aminolevulinic-acid-requiring Saccharomyces cerevisiae mutant.

Gene ·Vol. 28 ·No. 2 ·1984-05-00 ·Pages 195-9

Bard M, Ingolia TD

Abstract

Recombinant plasmids able to complement the Saccharomyces cerevisiae ole3 mutation were isolated. The nucleotide sequence responsible for complementation was localized to a 3.5-kb region. The level of delta-aminolevulinate (ALV) synthase activity in wild-type cells was six-fold lower than in plasmid-transformed ole3 mutant cells. Certain clones secreted a compound that supported growth of a lawn of adjacent ole3 mutant cells.

MeSH Terms

5-Aminolevulinate Synthetase/genetics,metabolism Aminolevulinic Acid/metabolism Base Sequence Chromosome Mapping DNA, Bacterial/genetics DNA, Fungal/genetics DNA, Recombinant Escherichia coli/genetics Genes, Fungal Genetic Complementation Test Levulinic Acids/metabolism Mutation Plasmids Saccharomyces cerevisiae/enzymology,genetics,growth & development Transformation, Genetic

Chemicals

DNA, Bacterial DNA, Fungal DNA, Recombinant Levulinic Acids Aminolevulinic Acid 5-Aminolevulinate Synthetase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Bard M

Ingolia T D

Article Info

Journal

Gene

Abbr.

Gene

ISSN

0378-1119

DOI

10.1016/0378-1119(84)90256-7

Published

1984-05-00

Pages

195-9

Language

English

Region

Netherlands

NLM ID

7706761

Subset

External Links

PubMed source DOI: 10.1016/0378-1119(84)90256-7 View journal details

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Plasmid-mediated complementation of a delta-aminolevulinic-acid-requiring Saccharomyces cerevisiae mutant.

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