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PMID: 6379605 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the rpoA-rplQ DNA of Escherichia coli: a second regulatory binding site for protein S4?

Nucleic acids research ·Vol. 12 ·No. 14 ·1984-07-25 ·Pages 5813-21

Meek DW, Hayward RS

Abstract

The "alpha-operon" of E.coli is a unit of regulation comprising the following known genes, mostly encoding ribosomal proteins (in order of transcription, and with their products named in brackets): rpsM (S13), rpsK (S11), rpsD (S4), rpoA (alpha-subunit of RNA polymerase), rplQ (L17). There is evidence that S4 tightly regulates all of these genes, except rpoA, by repressing translation of the polycistronic mRNA. Binding of S4 to the S13 start-site is thought to regulate the first three genes. We have extended the 'rpsD-rpoA' sequences previously determined by others, to include all of rpoA and rplQ. The rpoA-rplQ intercistronic region shows strong primary, and potential secondary structural homologies with the S4-binding sites on 16S rRNA and S13 mRNA. We suggest that S4 represses L17 translation directly.

MeSH Terms
Bacterial Proteins/genetics Base Sequence Binding Sites Escherichia coli/genetics Gene Expression Regulation Genes, Bacterial Nucleic Acid Conformation Protein Biosynthesis Ribosomal Proteins/genetics
Chemicals
Bacterial Proteins Ribosomal Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Meek D W
Hayward R S
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22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.14.5813
Published
1984-07-25
Pages
5813-21
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320033
Subset
IM
Databases
GENBANK
J01685, M11148, X00766
External Links
PubMed source DOI: 10.1093/nar/12.14.5813 PMC Full Text View journal details
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