Kinetics of the 580-nm ultrafast bacteriorhodopsin transient.

Biophysical journal ·Vol. 22 ·No. 1 ·1978-04-00 ·Pages 121-4

Kaufmann KJ, Sundstrom V, Yamane T, Rentzepis PM

Abstract

We have observed, by low-temperature picosecond spectroscopy, a photo-induced transient at 580 nm in light-adapted bacteriorhodopsin. The transient was characterized by bleaching in the 550-585-nm regions within 6 ps and recovery in approximately 20 ps. The spectral intensity of the transient is found to be enhanced at lower temperatures, and the lifetime slightly elongated.

MeSH Terms

Bacteriorhodopsins Carotenoids Kinetics Photochemistry Spectrophotometry

Chemicals

Carotenoids Bacteriorhodopsins

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Kaufmann K J

Sundstrom V

Yamane T

Rentzepis P M

References (8)

8 references, click to expand

Identification of retinal isomers isolated from bacteriorhodopsin.
Biochemistry. 1977 May 3;16(9):1955-9 PMID: 870032
The quantum efficiency of the bacteriorhodopsin photocycle.
Biophys J. 1977 Feb;17(2):179-83 PMID: 836935
A method for measuring picosecond phenomena in photolabile species: the emission lifetime of bacteriorhodopsin.
Biophys J. 1976 Dec;16(12):1399-409 PMID: 990393
Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane.
Methods Enzymol. 1974;31:667-78 PMID: 4418026
Observation of light emission from a rhodopsin.
Nature. 1976 Apr 22;260(5553):675-8 PMID: 1264238
Primary photochemical processes in bacteriorhodopsin.
Biochem Biophys Res Commun. 1976 Feb 23;68(4):1109-15 PMID: 1267768
Picosecond kinetics of the fluorescence from the chromophore of the purple membrane protein of Halobacterium halobium.
Biophys J. 1976 May;16(5):541-5 PMID: 1276383
Bacteriorhodopsin: a light-driven proton pump in Halobacterium Halobium.
Biophys J. 1975 Sep;15(9):955-62 PMID: 1182271

Article Info

Journal

Biophysical journal

Abbr.

Biophys J

ISSN

0006-3495

DOI

10.1016/S0006-3495(78)85476-9

Published

1978-04-00

Pages

121-4

Language

English

Region

United States

NLM ID

0370626

PMCID

PMC1473399

Subset

External Links

PubMed source DOI: 10.1016/S0006-3495(78)85476-9 PMC Full Text View journal details

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Kinetics of the 580-nm ultrafast bacteriorhodopsin transient.

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