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PMID: 6389341 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Monoclonal antibody detection of IncF group plasmid-encoded TraT protein in clinical isolates of Escherichia coli.

Infection and immunity ·Vol. 46 ·No. 2 ·1984-11-00 ·Pages 308-13

Bitter-Suermann D, Peters H, Jürs M, Nehrbass R, Montenegro M, Timmis KN

Abstract

The TraT protein specified by IncF group plasmids mediates surface exclusion and bacterial resistance to the lethal activities of serum. In this study, an anti-TraT protein monoclonal antibody was generated which failed to react with TraT+ bacteria but which efficiently detected solubilized TraT protein in Western blots and in an enzyme-linked immunosorbent assay. Use of this antibody to screen clinical and nonclinical isolates of Escherichia coli for the production of TraT protein revealed its presence in a modest proportion (38%) of normal fecal strains, a significantly higher proportion of clinical strains (51 to 73%), and an even higher proportion (78 to 88%) of clinical strains concomitantly producing the K1 capsule, an important virulence factor of E. coli.

MeSH Terms
Antibodies, Monoclonal/immunology Antigens, Bacterial/analysis Bacterial Outer Membrane Proteins/genetics,immunology Escherichia coli/immunology,pathogenicity Escherichia coli Infections/immunology Humans Plasmids
Chemicals
Antibodies, Monoclonal Antigens, Bacterial Bacterial Outer Membrane Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Bitter-Suermann D
Peters H
Jürs M
Nehrbass R
Montenegro M
Timmis K N
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20 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.46.2.308-313.1984
Published
1984-11-00
Pages
308-13
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC261531
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.46.2.308-313.1984 PMC Full Text View journal details
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