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PMID: 6389361 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A plasmid-encoded outer membrane protein, TraT, enhances resistance of Escherichia coli to phagocytosis.

Infection and immunity ·Vol. 46 ·No. 3 ·1984-12-00 ·Pages 740-6

Agüero ME, Aron L, DeLuca AG, Timmis KN, Cabello FC

Abstract

The presence of the outer membrane protein TraT, encoded by plasmid R6-5, reduces the sensitivity of Escherichia coli cells to phagocytosis by macrophages. This effect is independent of the bacterial capsule and is more evident in the presence of adsorbed normal human serum. The property of inhibiting phagocytosis is specifically abolished by anti-TraT protein antiserum and anti-TraT immunoglobulin G but not by Fab fragments. These results indicate that the TraT protein is a passive inhibitor of phagocytosis. Inhibition of phagocytosis is produced because the TraT protein antagonizes opsonization by complement, such that C3 deposition is reduced and altered in distribution.

MeSH Terms
Antibodies, Bacterial/immunology Bacterial Outer Membrane Proteins/genetics,immunology,pharmacology Blood Bactericidal Activity Complement Pathway, Alternative Escherichia coli/immunology Genes, Bacterial Immunoglobulin Fab Fragments/immunology Opsonin Proteins Phagocytosis/drug effects Plasmids
Chemicals
Antibodies, Bacterial Bacterial Outer Membrane Proteins Immunoglobulin Fab Fragments Opsonin Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Agüero M E
Aron L
DeLuca A G
Timmis K N
Cabello F C
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39 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.46.3.740-746.1984
Published
1984-12-00
Pages
740-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC261607
Subset
IM
Grants
NIAID NIH HHS · R01 AI10678 · United States
External Links
PubMed source DOI: 10.1128/iai.46.3.740-746.1984 PMC Full Text View journal details
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