A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.

Molecular & general genetics : MGG ·Vol. 197 ·No. 2 ·1984-00-00 ·Pages 345-6

Boeke JD, LaCroute F, Fink GR

Abstract

Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3- mutants grow normally. This selection, based on the loss of orotidine-5'-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells.

MeSH Terms

Carboxy-Lyases/genetics Drug Resistance, Microbial Fungal Proteins/genetics Microbiological Techniques Orotic Acid/analogs & derivatives,pharmacology Orotidine-5'-Phosphate Decarboxylase/genetics Saccharomyces cerevisiae/drug effects,enzymology,genetics

Chemicals

Fungal Proteins Orotic Acid 5-fluoroorotic acid Carboxy-Lyases Orotidine-5'-Phosphate Decarboxylase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Boeke J D

LaCroute F

Fink G R

References (10)

10 references, click to expand

Regulation of orotidylic acid pyrophosphorylase in Saccharomyces cerevisiae.
J Bacteriol. 1972 Jan;109(1):196-202 PMID: 4550660
Selection of lys2 Mutants of the Yeast SACCHAROMYCES CEREVISIAE by the Utilization of alpha-AMINOADIPATE.
Genetics. 1979 Sep;93(1):51-65 PMID: 17248969
Structure and function of the yeast URA3 gene: expression in Escherichia coli.
Gene. 1984 Jul-Aug;29(1-2):113-24 PMID: 6092217
Genetic and physiological characterization of met15 mutants of Saccharomyces cerevisiae: a selective system for forward and reverse mutations.
Genetics. 1975 Sep;81(1):75-97 PMID: 1107143
Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae.
Genetics. 1984 Jun;107(2):179-97 PMID: 6329902
Characteristics and relationships of mercury-resistant mutants and methionine auxotrophs of yeast.
J Bacteriol. 1974 Jun;118(3):911-8 PMID: 4364332
Identification of a Ty insertion within the coding sequence of the S. cerevisiae URA3 gene.
Mol Gen Genet. 1984;193(3):557-60 PMID: 6323928
Evidence for transcriptional regulation of orotidine-5'-phosphate decarboxylase in yeast by hybridization of mRNA to the yeast structural gene cloned in Escherichia coli.
Proc Natl Acad Sci U S A. 1979 Jan;76(1):386-90 PMID: 370827
Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments.
Gene. 1979 Dec;8(1):17-24 PMID: 395030
Direct selective techniques for the isolation of pyrimidine auxotrophs in yeast.
Mol Gen Genet. 1972;115(2):126-30 PMID: 4554474

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00330984

Published

1984-00-00

Pages

345-6

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00330984

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A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.

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