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PMID: 6401995 Published · ppublish English Case Reports Journal Article Research Support, Non-U.S. Gov't

Quantitation and detection of isotypes of anti-SS-B antibodies by ELISA and Farr assays using affinity purified antigens. An approach to the investigation of Sjögren's syndrome and systemic lupus erythematosus.

Arthritis and rheumatism ·Vol. 26 ·No. 2 ·1983-02-00 ·Pages 146-55

Venables PJ, Charles PJ, Buchanan RR, Yi T, Mumford PA, Schrieber L, Room GR, Maini RN

Abstract

Affinity purified SS-B was characterized as a protein with immunoreactive polypeptides of 40K and 29K. A modified Farr assay and an enzyme linked immunosorbent assay (ELISA) were 100- to 1,000-fold more sensitive than immunodiffusion and showed an association with the systemic manifestations of primary sicca syndrome and Sjögren's syndrome with systemic lupus erythematosus. The ELISA was sufficiently sensitive to detect class specific antibodies in saliva and lymphocyte culture supernatants.

MeSH Terms

Adult Antibodies, Antinuclear/analysis Antigens/isolation & purification Autoantigens Chromatography, Affinity Enzyme-Linked Immunosorbent Assay Female Humans Immunodiffusion Immunoenzyme Techniques Lupus Erythematosus, Systemic/immunology Lymphocytes/immunology Middle Aged Radioimmunoassay/methods Rheumatoid Factor/analysis Ribonucleoproteins Saliva/immunology Sjogren's Syndrome/immunology

Chemicals

Antibodies, Antinuclear Antigens Autoantigens Ribonucleoproteins SS-B antibodies SS-B antigen Rheumatoid Factor

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Venables P J

Charles P J

Buchanan R R

Yi T

Mumford P A

Schrieber L

Room G R

Maini R N

Article Info

Journal

Arthritis and rheumatism

Abbr.

Arthritis Rheum

ISSN

0004-3591

DOI

10.1002/art.1780260205

Published

1983-02-00

Pages

146-55

Language

English

Region

United States

NLM ID

0370605

Subset

External Links

PubMed source DOI: 10.1002/art.1780260205 View journal details

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Quantitation and detection of isotypes of anti-SS-B antibodies by ELISA and Farr assays using affinity purified antigens. An approach to the investigation of Sjögren's syndrome and systemic lupus erythematosus.

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