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PMID: 6403208 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A model aerosol exposure system for induction of porcine Haemophilus pleuropneumonia.

Canadian journal of comparative medicine : Revue canadienne de medecine comparee ·Vol. 47 ·No. 1 ·1983-01-00 ·Pages 48-53

Sebunya TN, Saunders JR, Osborne AD

Abstract

One group of six pigs and another group of three pigs were separately exposed in a polyethylene enclosed chamber for ten minutes, respectively, to Haemophilus pleuropneumoniae serotype 1 and Bacillus subtilis aerosols generated by an ultrasonic nebulizer. Haemophilus pleuropneumoniae and B. subtilis were deposited throughout the lungs immediately following aerosol exposure. The number of H. pleuropneumoniae and B. subtilis deposited varied within and between lungs in each group. The mean numbers of both organisms deposited in the posterior (caudal and accessory) lobes were significantly greater than those in the anterior (cranial and middle) lobes (P less than 0.001). The four principals that received H. pleuropneumoniae aerosols and the two contact controls developed fatal fibrinous pneumonia which simulated that seen in natural infections. Since this exposure system consistently resulted in clinical disease it has good potential as a model for the study of pathogenesis of the disease and more specifically for the evaluation of vaccines.

MeSH Terms
Aerosols Animals Bacillus subtilis/isolation & purification Disease Models, Animal Haemophilus/isolation & purification Haemophilus Infections/microbiology,pathology,veterinary Lung/microbiology,pathology Pleuropneumonia/microbiology,pathology,veterinary Swine Swine Diseases/microbiology,pathology
Chemicals
Aerosols
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sebunya T N
Saunders J R
Osborne A D
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19 references, click to expand
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Article Info
Journal
Canadian journal of comparative medicine : Revue canadienne de medecine comparee
Abbr.
Can J Comp Med
ISSN
0008-4050
Published
1983-01-00
Pages
48-53
Language
English
Region
Canada
NLM ID
0151747
PMCID
PMC1235883
Subset
IM
External Links
PubMed source PMC Full Text
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