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PMID: 6408641 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Complete sequence of a chicken lambda light chain immunoglobulin derived from the nucleotide sequence of its mRNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 13 ·1983-07-00 ·Pages 4099-103

Reynaud CA, Dahan A, Weill JC

Abstract

Recombinant cDNA plasmids have been constructed from chicken spleen poly(A)-containing RNA. Two clones have been selected and provide the sequence determination of a chicken lambda immunoglobulin light chain: they include the complete variable, constant, and 3' untranslated regions of the chicken lambda light chain mRNA and part of the leader sequence. Comparison of the chicken light chain constant region with both human and mouse lambda constant sequences indicates 61% homology at the amino acid level. Unexpectedly, the chicken variable sequence is 53-63% homologous to human variable sequences when it is compared to the various lambda subgroups and only 42% homologous to the mouse V lambda 1 sequence. The degree of homology between the variable regions of these three species does not easily correlate with their phylogenetic relationship.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chickens Cloning, Molecular DNA/metabolism Humans Immunoglobulin Light Chains/genetics Immunoglobulin lambda-Chains/genetics Mice Plasmids Protein Biosynthesis RNA, Messenger/genetics Rabbits Reticulocytes/metabolism Species Specificity
Chemicals
Immunoglobulin Light Chains Immunoglobulin lambda-Chains RNA, Messenger DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Reynaud C A
Dahan A
Weill J C
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40 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.13.4099
Published
1983-07-00
Pages
4099-103
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC394208
Subset
IM
Databases
GENBANK
K00678
External Links
PubMed source DOI: 10.1073/pnas.80.13.4099 PMC Full Text View journal details
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