A new isotype sequence (V kappa 27) of the variable region of kappa-light chains from a mouse hybridoma-derived anti-(streptococcal group A polysaccharide) antibody containing an additional cysteine residue. Application of the dimethylaminoazobenzene isothiocyanate technique for the isolation of peptides.

The Biochemical journal ·Vol. 211 ·No. 1 ·1983-04-01 ·Pages 173-80

Chang JY, Herbst H, Aebersold R, Braun DG

Abstract

The first complete sequence of the variable region of a kappa-light chain (V kappa) from a mouse anti-(streptococcal group A polysaccharide) antibody (immunoglobulin 7S34.1) is reported. Immunoglobulin 7S34.1 was isolated from the ascitic fluid of hybridoma 7S34.1 previously cloned in vitro. A newly developed technique for the isolation of peptides by using pre-column formation of peptide derivatives with dimethylaminoazobenzene isothiocyanate also served to complete the sequence. The sequence of the variable region of the kappa-light chain of immunoglobulin 7S34.1 defines a new mouse V kappa isotype (V kappa 27) and is the first mouse immunoglobulin light-chain variable region to be shown to have an extra cysteine residue at position 48.

MeSH Terms

Amino Acid Sequence Animals Antibodies, Bacterial Antibodies, Monoclonal Binding Sites, Antibody Chromatography, High Pressure Liquid Hybridomas Immunoglobulin Light Chains Immunoglobulin Variable Region Immunoglobulin kappa-Chains Isothiocyanates Mice Peptide Fragments/analysis Polysaccharides, Bacterial/immunology Streptococcus pyogenes/immunology Thiocyanates p-Dimethylaminoazobenzene/analogs & derivatives

Chemicals

Antibodies, Bacterial Antibodies, Monoclonal Immunoglobulin Light Chains Immunoglobulin Variable Region Immunoglobulin kappa-Chains Isothiocyanates Peptide Fragments Polysaccharides, Bacterial Thiocyanates streptococcal polysaccharide group A 4-(N,N-dimethylaminoazobenzene)-4'-isothiocyanate p-Dimethylaminoazobenzene

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Chang J Y

Herbst H

Aebersold R

Braun D G

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28 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2110173

Published

1983-04-01

Pages

173-80

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1154341

Subset

External Links

PubMed source DOI: 10.1042/bj2110173 PMC Full Text View journal details

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