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PMID: 6409099 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sugar transport in rat liver lysosomes. Direct demonstration by using labelled sugars.

The Biochemical journal ·Vol. 212 ·No. 1 ·1983-04-15 ·Pages 211-8

Maguire GA, Docherty K, Hales CN

Abstract

Purified rat liver lysosomes ('tritosomes') were prepared from rats injected with Triton WR-1339. 2. The water space of tritosomes, measured by using [3H]water and [14C]sucrose, was 2.15 +/- 0.72 microliter/mg of protein (mean +/- S.E.M., n = 12). 3. Tritosomes, when compared with a crude preparation of normal lysosomes by an indirect method of study, showed sugar specificity but decreased stereospecificity of sugar uptake. 4. At 125 mM the relative rates of net uptake of D-[14C]ribose, D-[14C]- or D-[3H]glucose and 2-deoxy-D-[3H]glucose were the same as that inferred from the indirect study. 5. The entry of D-[3H]glucose into tritosomes showed concentration-dependence suggestive of saturation, with a Km of 48 +/- 18 mM (4). 6. D- and L-glucose, D-ribose, 2-deoxy-D-glucose and D-mannose competed with D-[14C]glucose or D-[14C]ribose for uptake. 7. Cytochalasin B inhibited D-[3H]glucose uptake. 8. Uptake of 1 mM-L-[14C]glucose was slower than for 1 mM-D-[14C]glucose. 9. It is concluded that a facilitated-diffusion transport system is present in purified rat liver lysosomes.

MeSH Terms
Acetylglucosaminidase/metabolism Animals Biological Transport/drug effects Carbohydrate Metabolism Carbon Radioisotopes Cytochalasin B/pharmacology Glucose/metabolism In Vitro Techniques Liver/enzymology,metabolism Lysosomes/enzymology,metabolism Rats Ribose/metabolism Tritium
Chemicals
Carbon Radioisotopes Tritium Cytochalasin B Ribose Acetylglucosaminidase Glucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Maguire G A
Docherty K
Hales C N
References (26)
26 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2120211
Published
1983-04-15
Pages
211-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1152031
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2120211 PMC Full Text View journal details
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