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PMID: 6412036 Published · ppublish fre Journal Article Research Support, Non-U.S. Gov't

[Genetic analysis of sacB, the structural gene of a secreted enzyme, levansucrase of Bacillus subtilis Marburg].

Analyse génétique de sacB, gène de structure d'une enzyme secrétée, la lévane-saccharase de Bacillus subtilis Marburg.

Molecular & general genetics : MGG ·Vol. 191 ·No. 1 ·1983-00-00 ·Pages 138-44

Steinmetz M, Le Coq D, Djemia HB, Gay P

Abstract

The structural gene sacB encoding B. subtilis levansucrase, a secreted enzyme, expresses in E. coli. E. coli hosts of the sacB gene are poisoned by sucrose. This property allowed a powerful selection of mutants affected in the cloned gene. The plasmidic mutations were readily introduced in the B. subtilis chromosome. Using a collection of plasmids bearing various deletions extending in sacB we developed a technique of deletion mapping based on plasmid integration in the chromosome of B. subtilis. A generalization of this technique is discussed.

MeSH Terms
Bacillus subtilis/genetics Escherichia coli/genetics Hexosyltransferases/genetics Phenotype Sucrose/pharmacology Transformation, Bacterial
Chemicals
Sucrose Hexosyltransferases levansucrase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Steinmetz M
Le Coq D
Djemia H B
Gay P
References (22)
22 references, click to expand
  1. Identification of the structural gene of levansucrase in Bacillus subtilis Marburg.
    Mol Gen Genet. 1974 Feb 6;128(3):213-21 PMID: 4209418
  2. Detection of replicational inceptor signals in IS5.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:173-6 PMID: 6271462
  3. Nucleotide sequence of the transposable DNA-element IS2.
    Nucleic Acids Res. 1979 Mar;6(3):1111-22 PMID: 375194
  4. Molecular cloning and expression of Bacillus licheniformis beta-lactamase gene in Escherichia coli and Bacillus subtilis.
    J Bacteriol. 1981 Jan;145(1):422-8 PMID: 6257638
  5. Pleiotropic mutations affecting sporulation conditions and the syntheses of extracellular enzymes in Bacillus subtilis 168.
    Biochimie. 1974;56(11-12):1481-9 PMID: 4219582
  6. IS4 at its chromosomal site in E. coli K12.
    Mol Gen Genet. 1980;178(2):285-91 PMID: 6248724
  7. The galactose operon of E. coli K-12. II. A deletion analysis of operon structure and polarity.
    Genetics. 1969 Jun;62(2):249-64 PMID: 4905872
  8. Cloning structural gene sacB, which codes for exoenzyme levansucrase of Bacillus subtilis: expression of the gene in Escherichia coli.
    J Bacteriol. 1983 Mar;153(3):1424-31 PMID: 6402497
  9. Insertional mutagenesis in Bacillus subtilis: mechanism and use in gene cloning.
    Gene. 1982 Oct;19(3):277-84 PMID: 6295881
  10. ON THE TOPOLOGY OF THE GENETIC FINE STRUCTURE.
    Proc Natl Acad Sci U S A. 1959 Nov;45(11):1607-20 PMID: 16590553
  11. Chromosomal location of a Bacillus subtilis DNA fragment uniquely transcribed by sigma-28-containing RNA polymerase.
    J Bacteriol. 1982 Nov;152(2):780-5 PMID: 6290451
  12. Cloning of the genes for penicillinase, penP and penI, of Bacillus licheniformis in some vector plasmids and their expression in Escherichia coli, Bacillus subtilis, and Bacillus licheniformis.
    J Bacteriol. 1981 Sep;147(3):776-86 PMID: 6268607
  13. Integration of IS3 into IS2 generates a short sequence duplication.
    Mol Gen Genet. 1979;177(1):85-9 PMID: 395417
  14. Chromosomal location of mutations affecting sucrose metabolism in Bacillus subtilis Marburg.
    Mol Gen Genet. 1972;118(2):135-60 PMID: 4628133
  15. IS1 is involved in deletion formation in the gal region of E. coli K12.
    Mol Gen Genet. 1975;137(1):17-28 PMID: 1101028
  16. [Phenotypic expression and genetic localization of mutations affecting maltose metabolism in Escherichia coli K 12].
    Ann Inst Pasteur (Paris). 1967 Jun;112(6):673-98 PMID: 4862550
  17. Nucleotide sequence of an insertion element, IS1.
    Proc Natl Acad Sci U S A. 1978 Feb;75(2):615-9 PMID: 273224
  18. Cloning and expression of a Bacillus coagulans amylase gene in Escherichia coli.
    Mol Gen Genet. 1982;186(4):507-11 PMID: 6182447
  19. Genetic analysis of the maltose A region in Escherichia coli.
    J Bacteriol. 1969 May;98(2):559-67 PMID: 4891257
  20. Isolation of temperature-sensitive mutants of R plasmid by in vitro mutagenesis with hydroxylamine.
    J Bacteriol. 1976 Sep;127(3):1561-3 PMID: 783149
  21. Mapping of mutations affecting synthesis of exocellular enzymes in Bacillus subtilis. Identity of the sacUh, amyB and pap mutations.
    Mol Gen Genet. 1976 Nov 17;148(3):281-5 PMID: 827683
  22. Mapping a cloned gene under sporulation control by inserttion of a drug resistance marker into the Bacillus subtilis chromosome.
    J Bacteriol. 1980 Apr;142(1):90-8 PMID: 6768719
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330901
Published
1983-00-00
Pages
138-44
Language
fre
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330901
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