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PMID: 6416164 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Simple and rapid method for isolating large plasmid DNA from lactic streptococci.

Applied and environmental microbiology ·Vol. 46 ·No. 3 ·1983-09-00 ·Pages 549-52

Anderson DG, McKay LL

Abstract

A procedure for the rapid isolation of plasmid DNA larger than 30 megadaltons from lactic streptococci is described. This protocol can be used on a preparative scale to isolate sufficient quantities of plasmid DNA required for restriction analysis, cloning, or transformation experiments. A scaled-down protocol is very useful for rapidly screening the plasmid content of streptococcal strains. With this methodology, previously undetected large plasmids were observed.

MeSH Terms
DNA, Bacterial/isolation & purification Lactococcus lactis/genetics Methods Plasmids
Chemicals
DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Anderson D G
McKay L L
References (11)
11 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.46.3.549-552.1983
Published
1983-09-00
Pages
549-52
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC239314
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.46.3.549-552.1983 PMC Full Text View journal details
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