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PMID: 6416835 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The murine immunoglobulin alpha gene expresses multiple transcripts from a unique membrane exon.

The EMBO journal ·Vol. 2 ·No. 6 ·1983-00-00 ·Pages 887-98

Word CJ, Mushinski JF, Tucker PW

Abstract

Immunoglobulin heavy chains of each class exist in two forms, membrane (m) and secreted (s), which have different carboxy-terminal sequences encoded by different gene segments. We have sequenced the DNA spanning the coding region for the carboxy-terminal end of the alpha m chain in a BALB/c genomic clone and have examined alpha mRNA expression in transformed and normal B cells. Unlike the other murine heavy chain genes, which have two membrane exons (M1 and M2), the alpha gene has only one membrane exon (alpha M1). This unusual exon encodes a highly conserved transmembranal peptide plus flanking amino acids singular to IgA. The complexity of the alpha gene transcription is also unusual. Downstream of alpha M1, three AATAAA hexanucleotides are used to terminate as many as six distinct alpha membrane mRNAs. These appear to differ only in length and splicing pattern of their 3'-untranslated regions, and thus, encode the same alpha m protein. The alpha s protein is approximately 4000 daltons smaller than alpha m as judged by in vitro translation and is coded by a single 2.0-kb alpha s mRNA. We propose that the expression of alpha s and the multiple alpha m mRNAs is differentially controlled at the level of transcriptional termination.

MeSH Terms
Animals B-Lymphocytes/immunology Base Sequence Cell Membrane/immunology Cloning, Molecular Gene Expression Regulation Genes Immunoglobulin Heavy Chains/genetics Immunoglobulin alpha-Chains/genetics Mice Nucleic Acid Precursors/genetics Plasmacytoma/genetics,immunology RNA, Messenger/genetics Transcription, Genetic
Chemicals
Immunoglobulin Heavy Chains Immunoglobulin alpha-Chains Nucleic Acid Precursors RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Word C J
Mushinski J F
Tucker P W
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
887-98
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555205
Subset
IM
Grants
NCI NIH HHS · 5T32 CA 09082 · United States
NIAID NIH HHS · AI 18016 · United States
Databases
GENBANK
K00691
External Links
PubMed source PMC Full Text View journal details
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