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PMID: 6421738 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Conservation of peptide structure of outer membrane protein-macromolecular complex from Neisseria gonorrhoeae.

Infection and immunity ·Vol. 43 ·No. 3 ·1984-03-00 ·Pages 839-45

Hansen MV, Wilde CE

Abstract

The structural conservation of an outer membrane protein of Neisseria gonorrhoeae called OMP-MC (outer membrane protein-macromolecular complex) was investigated by determining the isoelectric point and amino-terminal amino acid sequence of the protein and by using high-performance liquid chromatography for comparative tryptic peptide mapping. The 76,000-dalton subunits generated by reduction and alkylation of the native 800,000-dalton complex from six test strains focused in ultrathin gels as bands of restricted heterogeneity at an approximate pI of 7.6. Dansyl chloride labeling indicated that all strains shared glycine as the amino-terminal amino acid. Sequence analysis of OMP-MC from two strains revealed no amino acid differences within the first 11 residues. Dual-label peptide maps revealed an extremely high degree of conservation of peptide structure. The results indicate that (i) OMP-MCs isolated from various strains of N. gonorrhoeae share structural homology and (ii) the 800,000-dalton complex is a homopolymer composed of 10 to 12 apparently identical 76,000-dalton subunits.

MeSH Terms
Amino Acid Sequence Bacterial Outer Membrane Proteins Chromatography, High Pressure Liquid Dansyl Compounds Isoelectric Focusing Macromolecular Substances Membrane Proteins/immunology,isolation & purification Neisseria gonorrhoeae/analysis Peptides
Chemicals
Bacterial Outer Membrane Proteins Dansyl Compounds Macromolecular Substances Membrane Proteins Peptides
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hansen M V
Wilde C E
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30 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.43.3.839-845.1984
Published
1984-03-00
Pages
839-45
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC264258
Subset
IM
Grants
NIAID NIH HHS · AI-16375 · United States
External Links
PubMed source DOI: 10.1128/iai.43.3.839-845.1984 PMC Full Text View journal details
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