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PMID: 6421803 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Isolation of Bacillus subtilis mutants pleiotropically insensitive to glucose catabolite repression.

Journal of bacteriology ·Vol. 157 ·No. 3 ·1984-03-00 ·Pages 942-4

Fisher SH, Magasanik B

Abstract

A pleiotropic mutant of Bacillus subtilis was isolated which overproduced in the presence of glucose several enzymes whose synthesis is subject to glucose catabolite repression. Examination of intracellular metabolites suggested that the mutation may have resulted in a defect in glycolysis, increasing phosphoenolpyruvate and decreasing pyruvate, 2-ketoglutarate, and oxaloacetate.

MeSH Terms
Aconitate Hydratase/biosynthesis Bacillus subtilis/enzymology,genetics,isolation & purification Enzyme Repression Genes, Bacterial Glucose/metabolism Glycolysis Histidine Ammonia-Lyase/biosynthesis Mutation Phosphoenolpyruvate/metabolism Pyruvates/metabolism Pyruvic Acid Suppression, Genetic alpha-Glucosidases/biosynthesis
Chemicals
Pyruvates Phosphoenolpyruvate Pyruvic Acid alpha-Glucosidases Aconitate Hydratase Histidine Ammonia-Lyase Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fisher S H
Magasanik B
References (10)
10 references, click to expand
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  8. Bacillus subtilis glutamine synthetase mutants pleiotropically altered in glucose catabolite repression.
    J Bacteriol. 1984 Feb;157(2):612-21 PMID: 6141156
  9. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.157.3.942-944.1984
Published
1984-03-00
Pages
942-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215352
Subset
IM
Grants
NIAID NIH HHS · 5-F32-AI05711 · United States
NIADDK NIH HHS · AM-13894 · United States
NIGMS NIH HHS · GM-07446 · United States
External Links
PubMed source DOI: 10.1128/jb.157.3.942-944.1984 PMC Full Text View journal details
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