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PMID: 6421824 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Studies on the expression of the microtubule-associated protein, tau, during mouse brain development, with newly isolated complementary DNA probes.

The Journal of cell biology ·Vol. 98 ·No. 3 ·1984-03-00 ·Pages 1090-7

Drubin DG, Caput D, Kirschner MW

Abstract

Tau protein is a collection of closely related polypeptides that associate with microtubules in vivo and stimulate their assembly in vitro. Using an affinity-purified antiserum against bovine brain tau protein, we found that the number and amount of tau polypeptides changes dramatically during mouse brain development. The different forms appear to result from changes in tau mRNA since in vitro translation products reflect the qualitative and quantitative changes found in vivo. To study the mRNA and genomic complexity of tau protein, we used tau mRNA, purified from polysomes with tau antiserum, to isolate embryonic mouse tau complementary DNA clones. With these probes we have determined that embryonic tau protein is translated from a 6-kb mRNA that persists throughout brain development.

MeSH Terms
Animals Brain/growth & development Cattle DNA/genetics Genes Mice Microtubules/metabolism Molecular Weight Nerve Tissue Proteins/genetics,metabolism Protein Biosynthesis Proteins/classification,genetics,metabolism RNA, Messenger/genetics tau Proteins
Chemicals
Nerve Tissue Proteins Proteins RNA, Messenger tau Proteins DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Drubin D G
Caput D
Kirschner M W
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31 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.98.3.1090
Published
1984-03-00
Pages
1090-7
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113151
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.98.3.1090 PMC Full Text View journal details
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