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PMID: 6436018 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Long identical repeats in the mouse gamma 2b switch region and their implications for the mechanism of class switching.

The EMBO journal ·Vol. 3 ·No. 9 ·1984-09-00 ·Pages 2033-40

Wu TT, Reid-Miller M, Perry HM, Kabat EA

Abstract

A computer sorting method was used to construct a dictionary by which long identical repeats of nucleotides could be identified and all available sequences of immunoglobulin switch regions were examined. The genomic mouse gamma 2b switch region contains two sets of four long identical repeats comprising 102, 72, 98 and 109 nucleotides respectively. The first is separated from the second by 347 nucleotides which contain the first 46 nucleotides of the 98 nucleotide set as a third partial repeat. These sets overlap the 49-bp separation between identical five nucleotide repeats GAGCT, GGGGT, ACCAG and CGAGC. Switches from S mu to S gamma 2b in and between these sets involve deletions of all or part of a set. Frequencies and locations of short repeats show differences between S mu, S epsilon, and S alpha and the other switch regions; these could determine specificity and locations of switches.

MeSH Terms
Animals Base Composition Base Sequence Cloning, Molecular Computers Genes Immunoglobulin Heavy Chains/genetics Immunoglobulin Variable Region/genetics Immunoglobulin gamma-Chains/genetics Mice Recombination, Genetic Repetitive Sequences, Nucleic Acid
Chemicals
Immunoglobulin Heavy Chains Immunoglobulin Variable Region Immunoglobulin gamma-Chains
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wu T T
Reid-Miller M
Perry H M
Kabat E A
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31 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-09-00
Pages
2033-40
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557640
Subset
IM
Grants
NIAID NIH HHS · 1 R01 AI19042 · United States
NCI NIH HHS · CA 13696 · United States
NCRR NIH HHS · N01-RR-8-2118 · United States
External Links
PubMed source PMC Full Text View journal details
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