The interaction of ethidium with synthetic double-stranded polynucleotides at low ionic strength.

Nucleic acids research ·Vol. 5 ·No. 1 ·1978-01-00 ·Pages 161-71

Baguley BC, Falkenhaug EM

Abstract

The interaction of ethidium with synthetic DNA and RNA double-stranded polymers at 0.01 M ionic strength, pH 7.0, has been studied by fluorimetry at low drug to nucleotide ratios. Binding constants have been calculated assuming an excluded-neighbouring site model for the interaction of ethidium with double-stranded polymers. The values obtained are poly d(AT).poly d(AT), 9.5 X 10(6) M-1; poly dA.poly dT, 6.5 X 10(5) M-1; poly d(GC).poly d(GC), 9.9 X 10(6) M-1; poly dG,poly dC, 4.5 X 1-(6) M-1; poly d(AC); poly d(GT), 9.8 X 10(6) M-1; poly d(AG).poly d(CT), 1.3 X 10(6) M-1; poly rA.poly rU, 4.1 X 10(7) M-1. The displacement of ethidium from poly d(AT).poly d(AT) by 9-aminoacridine and an acridine-containing antitumor agent (NSC 156303; 4'-(9-acridinylamino)methanesulphon-m-anisidide) has also been examined.

MeSH Terms

Acridines Binding, Competitive Ethidium Kinetics Osmolar Concentration Polydeoxyribonucleotides Polyribonucleotides Spectrometry, Fluorescence

Chemicals

Acridines Polydeoxyribonucleotides Polyribonucleotides Ethidium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Baguley B C

Falkenhaug E M

References (16)

16 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/5.1.161

Published

1978-01-00

Pages

161-71

Language

English

Region

England

NLM ID

0411011

PMCID

PMC341968

Subset

External Links

PubMed source DOI: 10.1093/nar/5.1.161 PMC Full Text View journal details

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The interaction of ethidium with synthetic double-stranded polynucleotides at low ionic strength.

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