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PMID: 6438526 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of active human factor VIII from recombinant DNA clones.

Nature ·Vol. 312 ·No. 5992 ·1984-00-00 ·Pages 330-7

Wood WI, Capon DJ, Simonsen CC, Eaton DL, Gitschier J, Keyt B, Seeburg PH, Smith DH, Hollingshead P, Wion KL, Delwart E, Tuddenham EG, Vehar GA, Lawn RM

Abstract

DNA clones encoding the complete 2,351 amino acid sequence for human factor VIII have been isolated and used to produce biologically active factor VIII in cultured mammalian cells. The recombinant protein corrects the clotting time of plasma from haemophiliacs and has many of the biochemical and immunological characteristics of serum-derived factor VIII.

MeSH Terms

Animals Base Sequence Cells, Cultured Cloning, Molecular Cricetinae DNA/genetics DNA, Recombinant Factor VIII/genetics Female Gene Expression Regulation Genes Hemophilia A/therapy Humans Molecular Sequence Data RNA, Messenger/genetics X Chromosome

Chemicals

DNA, Recombinant RNA, Messenger Factor VIII DNA

Authors & Affiliations

14 authors, click to expand affiliations / ORCID

Wood W I

Capon D J

Simonsen C C

Eaton D L

Gitschier J

Keyt B

Seeburg P H

Smith D H

Hollingshead P

Wion K L

Delwart E

Tuddenham E G

Vehar G A

Lawn R M

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/312330a0

Published

1984-00-00

Pages

330-7

Language

English

Region

England

NLM ID

0410462

Subset

Databases

GENBANK

X01179

External Links

PubMed source DOI: 10.1038/312330a0 View journal details

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Expression of active human factor VIII from recombinant DNA clones.

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