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PMID: 6441149 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effects of divalent cations on acetylcholine-evoked membrane potential in the ionophore A23187 treated mouse pancreas.

Pflugers Archiv : European journal of physiology ·Vol. 402 ·No. 4 ·1984-12-00 ·Pages 465-72

Iwatsuki N

Abstract

Membrane potential and resistance were measured in isolated segments of mouse pancreas superfused in vitro using two intracellular microelectrodes. The acinar cells were stimulated by microionophoretic ACh application from an extracellular AChCl filled micropipette. The membrane depolarization and resistance reduction evoked by short pulses of ACh stimulation were abolished by treatment with A23187 containing Ca-free solution. The subsequent exposure of the A23187-treated tissue to a Ca or Sr containing solution restored the responses to short pulses of ACh. Addition of Ba, Co, or Mn instead of Ca had no such restorative effects. Mn or Co markedly suppressed the restorative effects of Ca or Sr. It is concluded that A23187 treatment causes depletion of intracellular Ca stores. The restorative effect of Ca or Sr indicates that short pulses of ACh stimulation evoke an increase in intracellular Ca or Sr concentration. Short pulses of ACh seem to release Ca from the stimulant-sensitive Ca pool and, in addition, to increase the membrane Ca permeability.

MeSH Terms
Acetylcholine/pharmacology Animals Barium/pharmacology Calcimycin/pharmacology Calcium/pharmacology Cations, Divalent/pharmacology Cobalt/pharmacology Dose-Response Relationship, Drug Electric Conductivity In Vitro Techniques Membrane Potentials/drug effects Mice Pancreas/drug effects,physiology Strontium/pharmacology Verapamil/pharmacology
Chemicals
Cations, Divalent Barium Calcimycin Cobalt Verapamil Acetylcholine Calcium Strontium
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Iwatsuki N
References (25)
25 references, click to expand
  1. Effects of the calcium ionophore A23187 on pancreatic acinar cell membrane potentials and amylase release.
    J Physiol. 1977 Jan;264(2):323-39 PMID: 320310
  2. Pancreatic acinar cells: acetylcholine-evoked electrical uncoupling and its ionic dependency.
    J Physiol. 1978 Jan;274:81-06 PMID: 625014
  3. Acetylcholine-like effects of intracellular calcium application in pancreatic acinar cells.
    Nature. 1977 Jul 14;268(5616):147-9 PMID: 593308
  4. Synthesis, intracellular transport and discharge of exportable proteins in the pancreatic acinar cell and other cells.
    Biol Rev Camb Philos Soc. 1978 May;53(2):211-354 PMID: 208670
  5. The effects of manganese, cobalt and calcium on amylase secretion and calcium homeostasis in rat pancreas.
    J Physiol. 1982 Feb;323:353-75 PMID: 6178819
  6. Electrical coupling and uncoupling of exocrine acinar cells.
    J Cell Biol. 1978 Nov;79(2 Pt 1):533-45 PMID: 569159
  7. Pancreatic acinar cells: electrophysiological evidence for stimulant-evoked increase in membrane calcium permeability in the mouse.
    J Physiol. 1980 Jun;303:61-72 PMID: 7431246
  8. Relationship between calcium release and potassium release in rat parotid gland.
    J Physiol. 1979 Jun;291:457-65 PMID: 480237
  9. Stimulus-permeability coupling: role of calcium in the receptor regulation of membrane permeability.
    Pharmacol Rev. 1978 Jun;30(2):209-45 PMID: 224401
  10. Single-channel currents in isolated patches of plasma membrane from basal surface of pancreatic acini.
    Nature. 1982 Sep 9;299(5879):159-61 PMID: 6287285
  11. Effects of intracellular EGTA injection on stimulant-evoked membrane potential and resistance changes in pancreatic acinar cells.
    Pflugers Arch. 1980 Jul;386(2):147-52 PMID: 6776482
  12. Calcium channel.
    Annu Rev Neurosci. 1981;4:69-125 PMID: 6261668
  13. Mouse pancreatic acinar cells: voltage-clamp study of acetylcholine-evoked membrane current.
    J Physiol. 1981 Sep;318:57-71 PMID: 7320904
  14. Effect of extracellular manganese on amylase release from dispersed pancreatic acini.
    Am J Physiol. 1981 Nov;241(5):G359-64 PMID: 6171168
  15. Ca2+ control of electrolyte permeability in plasma membrane vesicles from cat pancreas.
    J Membr Biol. 1979 Apr 12;46(1):41-70 PMID: 36481
  16. Effects of manganese and other agents on the calcium uptake that follows depolarization of squid axons.
    J Physiol. 1973 Jun;231(3):511-26 PMID: 4783095
  17. Regulation of pancreatic exocrine function in vitro: initial steps in the actions of secretagogues.
    Annu Rev Physiol. 1979;41:55-66 PMID: 219765
  18. Pancreatic acinar cells: ionic dependence of acetylcholine-induced membrane potential and resistance change.
    J Physiol. 1975 Jan;244(2):431-65 PMID: 1142124
  19. Ionophore A23187 can mimick the changes in membrane permeability that occur during acetylcholine-stimulation of pancreatic acinar secretion.
    Pflugers Arch. 1982 Jul;394(1):26-31 PMID: 6812017
  20. The role of calcium in pancreatic acinar cell stimulus-secretion coupling: an electrophysiological approach.
    Ann N Y Acad Sci. 1978 Apr 28;307:599-617 PMID: 360950
  21. Pancreatic acinar cells: the role of calcium in stimulus-secretion coupling.
    J Physiol. 1976 Jan;254(3):583-606 PMID: 815543
  22. Effect of A23187 on amylase release from dispersed acini prepared from guinea pig pancreas.
    Am J Physiol. 1980 May;238(5):G458-66 PMID: 6155082
  23. Pancreatic acinar cells: localization of acetylcholine receptors and the importance of chloride and calcium for acetylcholine-evoked depolarization.
    J Physiol. 1977 Aug;269(3):723-33 PMID: 894612
  24. Cholecystokinin activation of single-channel currents is mediated by internal messenger in pancreatic acinar cells.
    Nature. 1982 Nov 4;300(5887):61-3 PMID: 6290896
  25. Pancreatic acinar cells: the acetylcholine equilibrium potential and its ionic dependency.
    J Physiol. 1977 Aug;269(3):735-51 PMID: 894613
Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00583949
Published
1984-12-00
Pages
465-72
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00583949 View journal details
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