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PMID: 6444664 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Feedback suppression of the immune response in vitro. II. IgVH-restricted antibody-dependent suppression.

The Journal of experimental medicine ·Vol. 151 ·No. 3 ·1980-03-01 ·Pages 681-94

Zubler RH, Benacerraf B, Germain RN

Abstract

Feedback suppression of the primary humoral immune response to sheep erythrocytes (SRBC) in vitro was induced with cell-free supernate material derived from antigen-(SRBC) activated B (sIg+) cells. This soluble products bears Ig determinants and binds to the eliciting antigen (SRBC). The activity of this antibody in suppressing anti-SRBC plaque-forming cell responses is restricted to spleen cell cultures containing B cells sharing VH genes with the B cells producing the suppressive antibody. The anti-hapten (trinitrophenyl) response to derivatized SRBC is not affected by antigen-primed B cells or their products. These data are compatible with suppression being mediated by anti-antigen antibody, either (a) via blockade of different SRBC epitopes recognized by a limited set of B cell clones in each mouse strain, (b) via triggering of an anti-idiotypic response, either antibody or suppressor T cell in nature, restricted to activity in cultures containing B cells sharing VH structures with the original antibody, or (c) via interference by preformed antibody with T cell help directed at idiotype bearing B cells.

MeSH Terms
Animals B-Lymphocytes/immunology Binding Sites, Antibody Cells, Cultured Erythrocytes/immunology Feedback Immune Tolerance Immunoglobulin Allotypes/genetics Immunoglobulin Heavy Chains Immunoglobulin Idiotypes Immunoglobulin Variable Region Mice Solubility T-Lymphocytes, Regulatory/immunology
Chemicals
Immunoglobulin Allotypes Immunoglobulin Heavy Chains Immunoglobulin Idiotypes Immunoglobulin Variable Region
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zubler R H
Benacerraf B
Germain R N
References (30)
30 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.151.3.681
Published
1980-03-01
Pages
681-94
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2185808
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.151.3.681 PMC Full Text View journal details
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