Metabolic modulation of stoichiometry in a proton pump.

The current-voltage characteristics of the ATP-dependent proton pump in the plasma membrane of Neurospora have been explored under varied metabolic conditions imposed by mutation and by differential respiratory inhibition. The reversal potential, or presumed equilibrium potential, for the pump was observed at about -400 mV under energy-replete conditions, and at about -200 mV during a stable metabolic downshift of 55 percent. Steady-state levels of adenine nucleotides and inorganic phosphate, however, were not affected by this partial energy restriction, so that under both normal and restricted conditions the apparent free energy of ATP hydrolysis remained near -500 mV. The results suggest that a normal pump stoichiometry of 1 H+ extruded/1 ATP split is modified to 2 H+/1 ATP, by chronic energy restriction.