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PMID: 6447689 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

purF-lac fusion and direction of purF transcription in Escherichia coli.

Journal of bacteriology ·Vol. 143 ·No. 3 ·1980-09-00 ·Pages 1156-64

Smith JM, Gots JS

Abstract

The purF locus codes for the first enzyme, glutamine phosphoribosylpyrophosphate amidotransferase, of the purine biosynthetic pathway. A strain of Escherichia coli K-12 was isolated in which the lac structural genes were fused to the control region of the purF locus. This purF-lac fusion was shown to respond to purine-specific regulatory signals. A plaque-forming lambda transducing phage bearing this purF-lac fusion was isolated. This phage was used to genetically determine the direction of transcription for the pufF locus by two independent means. Results from both methods agreed that the direction of transcription of the purF locus was clockwise on the standard Escherichia coli K-12 genetic map.

MeSH Terms
Amidophosphoribosyltransferase/genetics Bacteriophage lambda Chromosomes, Bacterial Escherichia coli/genetics Genes Genes, Regulator Lac Operon Pentosyltransferases/genetics Recombination, Genetic Transcription, Genetic Transduction, Genetic
Chemicals
Pentosyltransferases Amidophosphoribosyltransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Smith J M
Gots J S
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23 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.143.3.1156-1164.1980
Published
1980-09-00
Pages
1156-64
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC294467
Subset
IM
Grants
NCI NIH HHS · CA 02790+ · United States
External Links
PubMed source DOI: 10.1128/jb.143.3.1156-1164.1980 PMC Full Text View journal details
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