Home LiteratureArticle Details
PMID: 6447872 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Promoter for the establishment of repressor synthesis in bacteriophage lambda.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 6 ·1980-06-00 ·Pages 3191-5

Schmeissner U, Court D, Shimatake H, Rosenberg M

Abstract

Transcription of the lambda repressor gene (cI) is positively regulated by the phage-encoded proteins cII and cIII. We have isolated and characterized the 5'-terminal region of this RNA and shown that it originates at a promoter (pE) located between genes cro and cII. The DNA sequence of this promoter shows little homology to other known promoters. Initiation of transcription from PE is abolished by the cis-dominant mutations cY; these mutations alter the "-10" and "-35" regions of the promoter. We propose that the "-35" region is the site of activation of PE, possibly via the direct interaction of protein cII.

MeSH Terms
Bacteriophage lambda/genetics Base Sequence Binding Sites Chromosome Mapping Cloning, Molecular Escherichia coli/genetics Genes, Regulator Mutation Operon Plasmids RNA Polymerase I/metabolism RNA, Messenger/isolation & purification Repressor Proteins/biosynthesis,genetics Transcription Factors/genetics Transcription, Genetic
Chemicals
RNA, Messenger Repressor Proteins Transcription Factors RNA Polymerase I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schmeissner U
Court D
Shimatake H
Rosenberg M
References (37)
37 references, click to expand
  1. The 5' terminal nucleotide of RNA from vesicular stomatitis virus defective interfering particles.
    Virology. 1977 Apr;77(2):863-6 PMID: 193268
  2. Rat insulin genes: construction of plasmids containing the coding sequences.
    Science. 1977 Jun 17;196(4296):1313-9 PMID: 325648
  3. A ribosome binding site from the pR RNA of bacteriophage lambda.
    J Mol Biol. 1977 Aug 25;114(4):559-68 PMID: 915942
  4. RNA polymerase binding sites in lambdaplac5 DNA.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4914-8 PMID: 270725
  5. Sequence of Cro gene of bacteriophage lambda.
    Nature. 1977 Nov 17;270(5634):274-5 PMID: 593347
  6. Nucleotide sequence of cro, cII and part of the O gene in phage lambda DNA.
    Nature. 1978 Mar 30;272(5652):410-4 PMID: 264238
  7. The relationship between function and DNA sequence in an intercistronic regulatory region in phage lambda.
    Nature. 1978 Mar 30;272(5652):414-23 PMID: 634366
  8. Mutants of bacteriophage lambda which do not requre the cIII gene for efficient lysogenization.
    Virology. 1978 Jul 15;88(2):199-212 PMID: 695324
  9. Location of the regulatory site for establishment of repression by bacteriophage lambda.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):150-4 PMID: 284327
  10. Interaction site of Escherichia coli cyclic AMP receptor protein on DNA of galactose operon promoters.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5090-4 PMID: 228278
  11. Convergent transcription in bacteriophage lambda: interference with gene expression.
    J Mol Biol. 1979 Sep 15;133(2):249-66 PMID: 161329
  12. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  13. DNA sequence of regulatory region for integration gene of bacteriophage lambda.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2477-81 PMID: 6446712
  14. Site-specific recombination functions of bacteriophage lambda: DNA sequence of regulatory regions and overlapping structural genes for Int and Xis.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2482-6 PMID: 6446713
  15. Structure and function of the cy control region of bacteriophage lambda.
    J Mol Biol. 1980 Apr;138(2):209-30 PMID: 6447790
  16. Control of transcription termination: a rho-dependent termination site in bacteriophage lambda.
    J Mol Biol. 1980 Apr;138(2):231-54 PMID: 6447791
  17. An improved method for thin-layer chromatography of nucleotide mixtures containing 32P-labelled orthophosphate.
    J Chromatogr. 1969 Mar 11;40(1):103-9 PMID: 5777609
  18. Effect of mutations in the c2 and c3 genes of bacteriophage lambda on macromolecular synthesis in infected cells.
    J Mol Biol. 1970 May 14;49(3):639-55 PMID: 5453348
  19. Control of lambda repressor synthesis.
    Proc Natl Acad Sci U S A. 1971 Sep;68(9):2185-9 PMID: 4943790
  20. Establishment and maintenance of repression by bacteriophage lambda: the role of the cI, cII, and c3 proteins.
    Proc Natl Acad Sci U S A. 1971 Sep;68(9):2190-4 PMID: 4943791
  21. Bidirectional transcription and the regulation of Phage lambda repressor synthesis.
    Proc Natl Acad Sci U S A. 1972 Nov;69(11):3156-60 PMID: 4508309
  22. Control of gene expression in bacteriophage lambda.
    Annu Rev Genet. 1973;7:289-324 PMID: 4593306
  23. Control of short leftward transcripts from the immunity and ori regions in induced coliphage lambda.
    Mol Gen Genet. 1973 Nov 22;126(4):275-90 PMID: 4784811
  24. The roles of the lambda c3 gene and the Escherichia coli catabolite gene activation system in the establishment of lysogeny by bacteriophage lambda.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):779-82 PMID: 4362632
  25. Developmental pathways for the temperate phage: lysis vs lysogeny,.
    Annu Rev Genet. 1972;6(0):157-90 PMID: 4604314
  26. Repressor synthesis in vivo after infection of E. coli by a prm - mutant of bacteriophage lambda.
    Virology. 1975 Jan;63(1):273-7 PMID: 1089337
  27. Positive and negative regulation by the cII and cIII gene products of bacteriophage lambda.
    Virology. 1975 Feb;63(2):484-91 PMID: 1114699
  28. Nucleotide sequence of an RNA polymerase binding site from the DNA of bacteriophage fd.
    Proc Natl Acad Sci U S A. 1975 Feb;72(2):737-41 PMID: 1054851
  29. Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):784-8 PMID: 1093168
  30. Recognition sequences of repressor and polymerase in the operators of bacteriophage lambda.
    Cell. 1975 Jun;5(2):109-13 PMID: 1095210
  31. Control of bacteriophage lambda repressor synthesis after phage infection: the role of the N, cII, cIII and cro products.
    J Mol Biol. 1975 Apr 5;93(2):267-88 PMID: 1152053
  32. Specific binding of CAP factor to lac promoter DNA.
    Nature. 1975 Aug 21;256(5519):672-4 PMID: 1097944
  33. Lambda cin-1, a new mutation which enhances lysogenization by bacteriophage lambda, and the genetic structure of the lambda cy region.
    Genetics. 1976 Mar 25;82(3):401-16 PMID: 773742
  34. 4S oop RNA is a leader sequence for the immunity-establishment transcription in coliphage lambda.
    Nature. 1976 Jul 8;262(5564):112-6 PMID: 934330
  35. Autoregulation and function of a repressor in bacteriophage lambda.
    Science. 1976 Oct 8;194(4261):156-61 PMID: 959843
  36. Cyclic adenosine 5'-monophosphate in Escherichia coli.
    Bacteriol Rev. 1976 Sep;40(3):527-51 PMID: 186018
  37. An electron microscopic comparison of transcription on linear and superhelical DNA.
    J Mol Biol. 1976 Jul 25;105(1):161-76 PMID: 792457
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.6.3191
Published
1980-06-00
Pages
3191-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349580
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.6.3191 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2