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PMID: 6448580 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Transduction of methicillin resistance in Staphylococcus aureus: recipient effectiveness and beta-lactamase production.

Antimicrobial agents and chemotherapy ·Vol. 18 ·No. 3 ·1980-09-00 ·Pages 424-32

Stewart GC, Rosenblum ED

Abstract

The effectiveness of Staphylococcus aureus strain 8325-4 as a recipient for the transduction of methicillin resistance requires the presence of a penicillinase plasmid but was found to be independent of the lysogenic state of the recipient. Effectiveness is conferred by the plasmid in either the autonomous or integrated states, although the transduction rate is higher in the former. Once established, the maintenance and expression of methicillin resistance were independent of continued carriage of the plasmid deoxyribonucleic acid. Analysis of penicillinase plasmid mutants indicated that beta-lactamase production was the plasmid function responsible for recipient effectiveness. Supportive evidence included the abrogation of recipient effectiveness by the beta-lactamase inhibitor clavulanic acid and the elimination of a plasmid requirement with recipient strains carrying a chromosomal beta-lactamase determinant. A possible role for beta-lactamase production in the transduction of methicillin resistance is discussed.

MeSH Terms
DNA, Bacterial/biosynthesis Enzyme Induction/drug effects Methicillin/pharmacology Penicillin Resistance Penicillinase/metabolism Staphylococcus Phages Staphylococcus aureus/drug effects,enzymology Transduction, Genetic beta-Lactamases/metabolism
Chemicals
DNA, Bacterial Penicillinase beta-Lactamases Methicillin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stewart G C
Rosenblum E D
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36 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.18.3.424
Published
1980-09-00
Pages
424-32
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC284017
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.18.3.424 PMC Full Text View journal details
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