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PMID: 6450866 Published · ppublish English Journal Article

Genetic fine structure of the pyrE region containing the genes for ribosomal proteins L28 and L33 in Escherichia coli.

Molecular & general genetics : MGG ·Vol. 179 ·No. 2 ·1980-00-00 ·Pages 311-7

Isono K, Schnier J, Kitakawa M

Abstract

Temperature-sensitive mutants harbouring alterations in ribosomal proteins L28 and L33 have been isolated and used in mapping the genes coding for the two proteins. It was found that they mapped very close to each other and near pyrE at 80.7 min on the E. coli genetic map. The genes affected by the mutations have been concluded to be the structural genes for proteins L28 (rpmB) and L33 (rpmG) by constructing merodiploids heterozygous for pyrE and for the two ribosomal proteins. Various transduction studies with P1kc phages indicate the gene order in this region to be (rpmB, rpmG)-pyrE-spoT-gltC.

MeSH Terms
Bacterial Proteins/genetics Bacteriophage lambda Chromosome Mapping Chromosomes, Bacterial Conjugation, Genetic Escherichia coli/genetics Genes Genetic Markers Ribosomal Proteins/genetics Transduction, Genetic
Chemicals
Bacterial Proteins Genetic Markers Ribosomal Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Isono K
Schnier J
Kitakawa M
References (19)
19 references, click to expand
  1. Cluster of ribosomal protein genes in Escherichia coli containing genes for proteins S6, S18, and L9.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):6163-7 PMID: 366612
  2. A transducing lambda phage carrying grpE, a bacterial gene necessary for lambda DNA replication, and two ribosomal protein genes, rpsP (S16) and rplS (L19).
    Mol Gen Genet. 1978 Oct 24;165(3):247-56 PMID: 368561
  3. A new ribosomal protein locus in Escherichia coli: the gene for protein S6 maps at 97 min.
    Mol Gen Genet. 1977 Jun 8;153(2):115-20 PMID: 329109
  4. The gene for ribosomal protein L25 (rplY) maps at 47.3 min near nalA in Escherichia coli K-12.
    Mol Gen Genet. 1979 Nov;176(3):313-8 PMID: 392236
  5. Mutants of Escherichia coli requiring methionine or vitamin B12.
    J Bacteriol. 1950 Jul;60(1):17-28 PMID: 15436457
  6. Identification and organization of ribosomal protein genes of Escherichia coli carried by lambdafus2 transducing phage.
    J Biol Chem. 1977 Oct 25;252(20):7323-36 PMID: 332690
  7. Mutations affecting the structural genes and the genes coding for modifying enzymes for ribosomal proteins in Escherichia coli.
    Mol Gen Genet. 1978 Sep 20;165(1):15-20 PMID: 362162
  8. Isolation and characterization of specialized transducing lambda phages carrying ribosomal protein genes of Escherichia coli.
    Mol Gen Genet. 1980;180(2):343-9 PMID: 6450872
  9. IMPROVED METHOD FOR THE ISOLATION OF THYMINE-REQUIRING MUTANTS OF ESCHERICHIA COLI.
    J Bacteriol. 1965 Aug;90:554-5 PMID: 14329476
  10. The control of ribonucleic acid synthesis in Escherichia coli. IV. Relevance of unusual phosphorylated compounds from amino acid-starved stringent strains.
    J Biol Chem. 1969 Jun 25;244(12):3133-41 PMID: 4893338
  11. Genetics of bacterial ribosomes.
    Annu Rev Genet. 1977;11:297-347 PMID: 339818
  12. Isolation and characterization of temperature-sensitive mutants of Escherichia coli with altered ribosomal proteins.
    Mol Gen Genet. 1976 Dec 22;149(3):297-302 PMID: 799248
  13. Genes coding for ribosomal proteins S15, L21, and L27 map near argG in Escherichia coli.
    J Bacteriol. 1979 Jun;138(3):832-8 PMID: 378941
  14. Cloning the spoT gene of Escherichia coli: identification of the spoT gene product.
    J Bacteriol. 1979 Mar;137(3):1100-10 PMID: 374338
  15. The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins.
    Biochemistry. 1969 Jul;8(7):2897-905 PMID: 4897206
  16. Recalibrated linkage map of Escherichia coli K-12.
    Bacteriol Rev. 1976 Mar;40(1):116-67 PMID: 773363
  17. Genes encoding ribosomal proteins S16 and L19 form a gene cluster at 56.4 min in Escherichia coli.
    Mol Gen Genet. 1978 Oct 24;165(3):265-8 PMID: 368562
  18. Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.
    Anal Biochem. 1970 Aug;36(2):401-12 PMID: 4916449
  19. Further temperature-sensitive mutants of Escherichia coli with altered ribosomal proteins.
    Mol Gen Genet. 1977 Apr 29;152(3):239-43 PMID: 327280
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00425458
Published
1980-00-00
Pages
311-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00425458
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