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PMID: 6458813 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Suppressor factor from a T cell hybrid inhibits delayed-type hypersensitivity responses to azobenzenearsonate.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 10 ·1981-10-00 ·Pages 6441-5

Whitaker RB, Nepom JT, Sy MS, Takaoki M, Gramm CF, Fox I, Germain RN, Nelles MJ, Greene MI, Benacerraf B

Abstract

By using polyethylene glycol 1540, BW5147 AKR T lymphoma cells were fused with splenocytes from A/J mice treated so as to induce suppressor T cells specific for azobenzenearsonate (ABA). Of 576 microwells originally seeded, 132 demonstrated growing cell clones, 4 of which produced an ABA-binding supernatant factor. When tested in vivo for suppression of delayed-type hypersensitivity to ABA, two of these cell lines, A4 and F12, were shown to produce suppressive supernatant factors. Fluorescence analysis of the F12 cells with appropriate antisera demonstrated this T cell hybrid to be Thy 1.2+, Lyt 1+,2-, and surface immunoglobulin negative, the surface marker phenotype of conventional ABA-specific suppressor T cells. This cloned suppressor cell line, F12, produces a culture supernatant factor that is suppressive at dilutions up to 1:100 and has provided material for genetic and immunochemical analysis.

MeSH Terms
Animals Azo Compounds/pharmacology Cell Line Clone Cells Female Hybridomas/drug effects,immunology Hypersensitivity, Delayed/immunology Immunosuppressive Agents/immunology Mice Mice, Inbred Strains Organic Chemicals T-Lymphocytes, Regulatory/drug effects,immunology p-Azobenzenearsonate/pharmacology
Chemicals
Azo Compounds Immunosuppressive Agents Organic Chemicals suppressive factor of allergy p-Azobenzenearsonate
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Whitaker R B
Nepom J T
Sy M S
Takaoki M
Gramm C F
Fox I
Germain R N
Nelles M J
Greene M I
Benacerraf B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.10.6441
Published
1981-10-00
Pages
6441-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349055
Subset
IM
Grants
NCI NIH HHS · 5T32-CA-09103-05 · United States
NIAID NIH HHS · AI-14732-04 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.10.6441 PMC Full Text View journal details
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