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PMID: 6461846 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Replication of lambda dv DNA in vitro.

Nucleic acids research ·Vol. 10 ·No. 5 ·1982-03-11 ·Pages 1733-40

Anderl A, Klein A

Abstract

Exogenous lambda dv DNA was replicated in extracts prepared from E. coli cells carrying plasmids with inducible lambda O and /or P genes. Extracts from cells carrying only one of the two lambda replication functions complement each other in this reaction. The reaction further requires ribonucleotide triphosphates, an ATP regenerating system, DNA gyrase and RNA polymerase functions. Density labelling of the superhelical reaction products results in hybrid density indicating that one complete round of replication has taken place in vitro.

MeSH Terms
Bacteriophage lambda/genetics DNA Replication Escherichia coli/genetics Genes, Viral Genetic Complementation Test Kinetics Plasmids Templates, Genetic Virus Replication
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Anderl A
Klein A
References (26)
26 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.5.1733
Published
1982-03-11
Pages
1733-40
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320562
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.5.1733 PMC Full Text View journal details
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