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PMID: 6461859 Published · ppublish English Journal Article

Characterization of a porcine genomic clone encoding a major histocompatibility antigen: expression in mouse L cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 5 ·1982-03-00 ·Pages 1403-7

Singer DS, Camerini-Otero RD, Satz ML, Osborne B, Sachs D, Rudikoff S

Abstract

A porcine genomic clone encoding a major histocompatibility, (MHC) antigen was isolated by direct screening of a swine genomic library with a heterologous human MHC cDNA probe. Mouse L cells transformed with DNA from the clone stably express swine MHC antigen. Pig alloantisera specifically lyse transformant but not control cell lines in a complement-mediated cytotoxicity assay. Direct immunoprecipitation of radiolabeled cellular protein from transformed lines by pig alloantiserum results in the coprecipitation of swine MHC heavy chain and mouse beta 2-microglobulin, demonstrating the association of heterologous subunits of MHC antigens.

MeSH Terms
Animals Bacteriophage lambda/genetics Cloning, Molecular Gene Expression Regulation Genes Histocompatibility Antigens/genetics L Cells Mice Nucleic Acid Hybridization Swine/genetics,immunology
Chemicals
Histocompatibility Antigens
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Singer D S
Camerini-Otero R D
Satz M L
Osborne B
Sachs D
Rudikoff S
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.5.1403
Published
1982-03-00
Pages
1403-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345981
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.5.1403 PMC Full Text View journal details
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