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PMID: 6462133 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The binding of human complement proteins C5, factor B, beta 1H and properdin to complement fragment C3b on zymosan.

The Biochemical journal ·Vol. 199 ·No. 3 ·1981-12-01 ·Pages 485-96

DiScipio RG

Abstract

The covalent binding of complement fragment C3b to zymosan by the action of the alternative-pathway C3 convertase and the reversible binding of several complement proteins (component C5, factor B, beta 1H and properdin) to C3b on zymosan have been investigated. When C3b is deposited on zymosan after activation by a surface-bound C3 convertase, the C3b molecules are deposited in foci around the C3 convertase site, with an average of 30 C3b molecules per site. The association constants of C5, factor B, beta 1H, and properdin for C3b bound to zymosan have been determined. The association constants ranged from 6.5 x 10(-5) M-1 for factor B to 2.9 x 10(7) M-1 for properdin. An approximate stoichiometry of 1 : 1 for C5, factor B, and properdin binding to C3b has been observed. Curvilinear Scatchard plots were observed for beta 1H binding to C3b, with the maximal extrapolated ratio of beta 1H to C3b of 0.32. Physiological amounts of properdin increase by 7-fold the affinity constant for factor B binding to C3b with no alteration in the stoichiometry. Similarly, physiological amounts of factor B increase the affinity constant of properdin to C3b about 4-fold with only a small measured difference in stoichiometry. Competition binding studies and protein modification suggest that C5, factor B, beta 1H, and properdin each bind to a distinct region on C3b.

MeSH Terms
Binding, Competitive Chloromercuribenzoates/pharmacology Complement C3b/metabolism Complement C3b Inactivator Proteins/metabolism Complement C5/metabolism Complement Factor B/metabolism Complement Factor H Dithiothreitol/pharmacology Enzyme Precursors/metabolism Humans Kinetics Properdin/metabolism Protein Binding/drug effects Zymosan/metabolism
Chemicals
CFH protein, human Chloromercuribenzoates Complement C3b Inactivator Proteins Complement C5 Enzyme Precursors Properdin Complement C3b Complement Factor H Zymosan Complement Factor B Dithiothreitol
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
DiScipio R G
References (49)
49 references, click to expand
  1. The binding of human complement component C4 to antibody-antigen aggregates.
    Biochem J. 1980 Jul 1;189(1):67-80 PMID: 6906229
  2. Evidence for presence of an internal thiolester bond in third component of human complement.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5764-8 PMID: 6934510
  3. Purification of the human complement control protein C3b inactivator.
    Biochem J. 1980 Oct 1;191(1):173-82 PMID: 6451220
  4. The separation of functionally distinct forms of the third component of human complement (C3).
    Biochem J. 1981 Mar 1;193(3):963-70 PMID: 6458280
  5. Covalent binding of proteinases in their reaction with alpha 2-macroglobulin.
    Biochem J. 1980 Jun 1;187(3):695-701 PMID: 6204637
  6. Ultrastructural localization of the third and fourth components of complement on complement-cell complexes.
    Am J Pathol. 1968 Aug;53(2):253-61 PMID: 5667579
  7. Receptors for complement of leukocytes.
    J Exp Med. 1968 Nov 1;128(5):991-1009 PMID: 4176227
  8. Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.
    J Biol Chem. 1970 Jun;245(12):3059-65 PMID: 5432796
  9. A population of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. I. Separation and characterization.
    J Exp Med. 1970 Oct 1;132(4):702-20 PMID: 4101362
  10. The reaction mechanism of human C5 in immune hemolysis.
    J Exp Med. 1970 Oct 1;132(4):775-93 PMID: 5508377
  11. C3 proactivator convertase and its mode of action.
    J Exp Med. 1972 Apr 1;135(4):1003-8 PMID: 4111773
  12. Solid state lactoperoxidase: a highly stable enzyme for simple, gentle iodination of proteins.
    Biochem Biophys Res Commun. 1972 Jul 25;48(2):464-71 PMID: 5065068
  13. Changes in antigenic properties of human C3 upon activation and conversion by trypsin.
    J Immunol. 1974 Apr;112(4):1444-51 PMID: 4205530
  14. Studies on the sub-unit structure of human properdin.
    Immunochemistry. 1974 Jul;11(7):361-8 PMID: 4477550
  15. Specificity of human lymphocyte complement receptors.
    J Exp Med. 1975 May 1;141(5):1163-80 PMID: 805205
  16. Third component of human complement: purification from plasma and physicochemical characterization.
    Biochemistry. 1976 Oct 5;15(20):4513-21 PMID: 823964
  17. Alternative pathway of complement: recruitment of precursor properdin by the labile C3/C5 convertase and the potentiation of the pathway.
    J Exp Med. 1976 Oct 1;144(4):1076-93 PMID: 978134
  18. Modulation of the alternative complement pathways by beta 1 H globulin.
    J Exp Med. 1976 Nov 2;144(5):1147-63 PMID: 62817
  19. A comparison of human prothrombin, factor IX (Christmas factor), factor X (Stuart factor), and protein S.
    Biochemistry. 1977 Feb 22;16(4):698-706 PMID: 836809
  20. Alternative pathway of complement: nonenzymatic, reversible transition of precursor to active properdin.
    J Immunol. 1977 Feb;118(2):525-32 PMID: 839069
  21. Complement activation by the properdin system: formation of a stoichiometric. C3 cleaving complex of properdin factor B with C36.
    Immunochemistry. 1977 Mar;14(3):201-5 PMID: 863465
  22. Receptor-binding sites on C3 and C3b.
    J Immunol. 1977 Jun;118(6):2145-50 PMID: 864256
  23. Human complement C3b inactivator: isolation, characterization, and demonstration of an absolute requirement for the serum protein beta1H for cleavage of C3b and C4b in solution.
    J Exp Med. 1977 Jul 1;146(1):257-70 PMID: 301546
  24. Interaction between the third complement protein and cell surface macromolecules.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):2701-5 PMID: 268620
  25. Chemical characterization of human factor B of the alternate pathway of complement activation.
    Biochemistry. 1977 Nov 29;16(24):5368-75 PMID: 411505
  26. Mechanism of action of the C3b inactivator: requirement for a high molecular weight cofactor (C3b-C4bINA cofactor) and production of a new C3b derivative (C3b').
    Immunochemistry. 1977 Nov-Dec;14(11-12):749-56 PMID: 146022
  27. A new function of the activated third component of complement: binding to C5, an essential step for C5 activation.
    Immunology. 1978 Jan;34(1):29-40 PMID: 624565
  28. Reaction mechanisms of beta1H globulin.
    Int Arch Allergy Appl Immunol. 1978;57(3):221-32 PMID: 77846
  29. Complement C3 convertase: cell surface restriction of beta1H control and generation of restriction on neuraminidase-treated cells.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2416-20 PMID: 276881
  30. Interaction of beta1H globulin with cell-bound C3b: quantitative analysis of binding and influence of alternative pathway components on binding.
    J Exp Med. 1978 Jun 1;147(6):1792-1805 PMID: 567241
  31. Human C4-binding protein. II. Role in proteolysis of C4b by C3b-inactivator.
    J Exp Med. 1978 Oct 1;148(4):1044-51 PMID: 702059
  32. Human alternative complement pathway: membrane-associated sialic acid regulates the competition between B and beta1 H for cell-bound C3b.
    J Immunol. 1979 Jan;122(1):75-81 PMID: 762425
  33. Interference of propamidine with binding of the fifth component of complement to surface-fixed C3b, and with C5 activation.
    Immunology. 1979 Jan;36(1):139-43 PMID: 422225
  34. Fifth component of human complement: purification from plasma and polypeptide chain structure.
    Biochemistry. 1979 Apr 17;18(8):1490-7 PMID: 106884
  35. Action of the C3b-inactivator on the cell-bound C3b.
    J Immunol. 1979 Mar;122(3):759-65 PMID: 448074
  36. Heat-induced fragmentation of human alpha 2-macroglobulin.
    J Biol Chem. 1979 Sep 10;254(17):8669-78 PMID: 89116
  37. Evidence for an ester linkage between the labile binding site of C3b and receptive surfaces.
    J Immunol. 1979 Sep;123(3):1388-94 PMID: 38283
  38. Characterization of alkylamine-sensitive site in alpha 2-macroglobulin.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4313-6 PMID: 92026
  39. The interaction of C5 with C3b in free solution: a sufficient condition for cleavage by a fluid phase C3/C5 convertase.
    J Immunol. 1980 Jan;124(1):326-31 PMID: 7350230
  40. Native and activated properdin: interconvertibility and identity of amino- and carboxy-terminal sequences.
    J Immunol. 1980 Feb;124(2):602-6 PMID: 6766162
  41. The temperature-sensitive bond in human alpha 2-macroglobulin is the alkylamine-reactive site.
    J Biol Chem. 1980 May 10;255(9):3820-3 PMID: 6154704
  42. Methylamine reaction and denaturation-dependent fragmentation of complement component 3. Comparison with alpha2-macroglobulin.
    J Biol Chem. 1980 Aug 10;255(15):7082-4 PMID: 6156166
  43. Cleavage of C4b by C3b inactivator: production of a nicked form of C4b, C4b', as an intermediate cleavage product of C4b by C3b inactivator.
    J Immunol. 1980 Aug;125(2):578-82 PMID: 7391570
  44. Interaction between the labile binding sites of the fourth (C4) and fifth (C5) human complement proteins and erythrocyte cell membranes.
    J Immunol. 1980 Aug;125(2):634-9 PMID: 7391573
  45. Third component of human complement: appearance of a sulfhydryl group following chemical or enzymatic inactivation.
    Biochemistry. 1980 Sep 16;19(19):4471-8 PMID: 7407085
  46. Effect of reduction on the structural, biologic, and immunologic properties of the third component of human complement.
    J Immunol. 1980 Sep;125(3):1099-103 PMID: 7410831
  47. The effects of mild reduction on the structure and function of C3.
    J Immunol. 1980 Oct;125(4):1798-805 PMID: 7410856
  48. Molecular biology and chemistry of the alternative pathway of complement.
    Adv Immunol. 1980;29:1-53 PMID: 6158260
  49. A thiol-ester in alpha 2-macroglobulin cleaved during proteinase complex formation.
    FEBS Lett. 1980 Dec 1;121(2):275-9 PMID: 6161841
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1990485
Published
1981-12-01
Pages
485-96
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1163402
Subset
IM
Grants
NIAID NIH HHS · F-32-AI-05808 ALY · United States
External Links
PubMed source DOI: 10.1042/bj1990485 PMC Full Text View journal details
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