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PMID: 6462917 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sequence of an expressed human beta-tubulin gene containing ten Alu family members.

Nucleic acids research ·Vol. 12 ·No. 14 ·1984-07-25 ·Pages 5823-36

Lee MG, Loomis C, Cowan NJ

Abstract

The complete sequence of a functionally expressed human beta-tubulin gene (5 beta) is presented. The amino acid sequence encoded by this gene constitutes a distinct isotype, differing from a previously described human beta-tubulin sequence at 21 positions throughout the polypeptide chain. The beta-tubulin coding sequence in 5 beta is interrupted by three intervening sequences of 1014, 117 and 4826 nucleotides. The largest of these contains ten members of the Alu family of middle repetitive sequences. Together, these regions account for sixty percent of this intervening sequence. Two of the Alu elements are juxtaposed head to tail, and share the same flanking direct repeat. The ten Alu sequences are substantially divergent, both from each other and from an Alu consensus sequence, and several contain deletions of up to half the entire sequence.

MeSH Terms
Amino Acid Sequence Base Sequence Gene Expression Regulation Genes Humans Repetitive Sequences, Nucleic Acid Tubulin/genetics
Chemicals
Tubulin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lee M G
Loomis C
Cowan N J
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35 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.14.5823
Published
1984-07-25
Pages
5823-36
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320034
Subset
IM
Grants
NIGMS NIH HHS · GM 31740 · United States
Databases
GENBANK
X00734
External Links
PubMed source DOI: 10.1093/nar/12.14.5823 PMC Full Text View journal details
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