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PMID: 647023 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Quantitation of submicrogram quantities of protein by an improved protein-dye binding assay.

Biochimica et biophysica acta ·Vol. 533 ·No. 2 ·1978-04-26 ·Pages 525-9

Bearden JC

Abstract

A quantitative assay method for protein is described which is based on the color change occurring in Coomassie Brilliant Blue G-250 when it binds to protein. This modification of two similar procedures further increases the sensitivity, simplicity, and stability of the protein-dye binding assay over those of other commonly used assays for protein.

MeSH Terms

Colorimetry/methods Indicators and Reagents Microchemistry Proteins/analysis

Chemicals

Indicators and Reagents Proteins

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Bearden J C

Article Info

Journal

Biochimica et biophysica acta

Abbr.

Biochim Biophys Acta

ISSN

0006-3002

DOI

10.1016/0005-2795(78)90398-7

Published

1978-04-26

Pages

525-9

Language

English

Region

Netherlands

NLM ID

0217513

Subset

External Links

PubMed source DOI: 10.1016/0005-2795(78)90398-7

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Quantitation of submicrogram quantities of protein by an improved protein-dye binding assay.

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