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PMID: 6477477 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Flow cytometry is a new method for the characterization of intestinal plasma membrane.

The Biochemical journal ·Vol. 221 ·No. 2 ·1984-07-15 ·Pages 453-7

Gorvel JP, Mawas C, Maroux S, Mishal Z

Abstract

The highly differentiated plasma membrane of rabbit enterocytes constitutes an interesting model for membrane studies. Flow cytometry allows combined measurements of the size of membrane vesicles by light-scatter and fluorescence polarization at a single-particle level. The degree of fluorescence polarization of 1,6-diphenylhexa-1,3,5-triene was determined at 4, 18 and 37 degrees C in the brush-border and basolateral plasma membranes. The fluorescence polarization was considerably higher in brush-border than in basolateral membranes. After incubation with dimethyl sulphoxide, the membrane fluidity decreased in both types of membranes. Moreover, a time-effect study of dimethyl sulphoxide showed changes in fluorescence polarization. Only in brush-border membrane a temporary fluid phase was observed. The different properties of the two membrane domains in relation to the lipid-protein dynamics of enterocytes are discussed.

MeSH Terms
Animals Cell Membrane/ultrastructure Cell Separation Dimethyl Sulfoxide/pharmacology Flow Cytometry/methods Fluorescence Polarization Intestine, Small/ultrastructure Light Membrane Fluidity/drug effects Microvilli/ultrastructure Rabbits Scattering, Radiation
Chemicals
Dimethyl Sulfoxide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gorvel J P
Mawas C
Maroux S
Mishal Z
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24 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2210453
Published
1984-07-15
Pages
453-7
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1144058
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2210453 PMC Full Text View journal details
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