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PMID: 6480561 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Monoclonal antibodies to Rhizobium meliloti and surface mutants insensitive to them.

Journal of bacteriology ·Vol. 160 ·No. 1 ·1984-10-00 ·Pages 454-7

Johansen E, Finan TM, Gefter ML, Signer ER

Abstract

Monoclonal antibodies were produced to the surface of the symbiotic nitrogen-fixing bacterium Rhizobium meliloti. Bacterial lysis in the presence of complement or cycles of agglutination and growth were used to select mutants no longer recognized by the antibodies. The mutants were used to produce new antibodies with different specificities. Several mutants had altered sensitivity to one or more bacteriophages. R. meliloti strains from different sources had distinct patterns of sensitivity to monoclonal antibodies and phages, which together can be used for discriminative typing.

MeSH Terms
Antibodies, Monoclonal Antigen-Antibody Complex Antigens, Bacterial/analysis,genetics Antigens, Surface/analysis,genetics Bacteriophages/genetics Mutation Rhizobium/genetics Species Specificity
Chemicals
Antibodies, Monoclonal Antigen-Antibody Complex Antigens, Bacterial Antigens, Surface
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Johansen E
Finan T M
Gefter M L
Signer E R
References (20)
20 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.160.1.454-457.1984
Published
1984-10-00
Pages
454-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214744
Subset
IM
Grants
NIAID NIH HHS · AI 13357-07 · United States
NIAID NIH HHS · AI 16873 · United States
NCI NIH HHS · CA 28900-03 · United States
External Links
PubMed source DOI: 10.1128/jb.160.1.454-457.1984 PMC Full Text View journal details
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