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PMID: 6484 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isocratic separation of some purine nucleotide, nucleoside, and base metabolites from biological extracts by high-performance liquid chromatography.

Journal of chromatography ·Vol. 121 ·No. 2 ·1976-06-23 ·Pages 251-62

Anderson FS, Murphy RC

Abstract

Separation of ATP, ADP, AMP, adenine, adenosine, cAMP, ITP, IDP, IMP, hypoxanthine, inosine, cIMP, the guanine series, NAD, NADPH, xanthine, 3-methylxanthine, theobromine, theophylline, and caffeine was accomplished using high-performance liquid chromatography with a microparticulate reversed-phase column. Under isocratic conditions all compounds could be eluted with reasonable resolution and retention time. Quantitation by peak height for several of the compounds was used to the 10-ng level.

MeSH Terms

Adenine Nucleotides/analysis Animals Blood Platelets/analysis Caffeine/analysis Cats Chromatography, High Pressure Liquid Erythrocytes/analysis Guanine/analysis Hypoxanthines/analysis Inosine/analysis Inosine Nucleotides/analysis NAD/analysis NADP/analysis Nucleosides/analysis Purine Nucleotides/analysis Spleen/analysis Theobromine/analysis

Chemicals

Adenine Nucleotides Hypoxanthines Inosine Nucleotides Nucleosides Purine Nucleotides NAD Caffeine NADP Inosine Guanine Theobromine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Anderson F S

Murphy R C

Article Info

Journal

Journal of chromatography

Abbr.

J Chromatogr

DOI

10.1016/s0021-9673(00)85021-9

Published

1976-06-23

Pages

251-62

Language

English

Region

Netherlands

NLM ID

0427043

Subset

External Links

PubMed source DOI: 10.1016/s0021-9673(00)85021-9

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Isocratic separation of some purine nucleotide, nucleoside, and base metabolites from biological extracts by high-performance liquid chromatography.

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