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PMID: 649658 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Proteins of rough microsomal membranes related to ribosome binding. I. Identification of ribophorins I and II, membrane proteins characteristics of rough microsomes.

The Journal of cell biology ·Vol. 77 ·No. 2 ·1978-05-00 ·Pages 464-87

Kreibich G, Ulrich BL, Sabatini DD

Abstract

Rat liver rough microsomes (RM) contain two integral membrane proteins which are not found in smooth microsomes (SM) and appear to be related to the presence of ribosome-binding sites. These proteins, of molecular weight 65,000 and 63,000, were designated ribophorins I and II, respectively. They were not released from the microsomal membranes by alkali or acid treatment, or when the ribosomes were detached by incubation with puromycin in a high salt medium. The anionic detergent sodium deoxycholate caused solubilization of the ribophorins, but neutral detergents led to their recovery with the sedimentable ribosomes. Ribosomal aggregates containing both ribophorins, but few other membrane proteins, were obtained from RM treated with the nonionic detergent Kyro EOB (2.5 X10(-2) M) in a low ionic strength medium. Sedimentation patterns produced by these aggregates resembled those of large polysomes but were not affected by RNase treatment. The aggregates, however, were dispersed by mild trypsinization (10 microgram trypsin for 30 min at 0 degrees C), incubation with deoxycholate, or in a medium of high salt concentration. These treatments led to a concomitant degradation or release of the ribophorins. It was estimated, from the staining intensity of protein bands in acrylamide gels, that in the Kyro EOB aggregates there were one to two molecules of each ribophorin per ribosome. Sedimentable complexes without ribosomes containing both ribophorins could also be obtained by dissolving RM previously stripped of ribosomes by puromycin-KCl using cholate, a milder detergent than DOC. Electron microscope examination of the residue obtained from RM treated with Kyro EOB showed that the rapidly sedimenting polysome-like aggregates containing the ribophorins consisted of groups of tightly packed ribosomes which were associated with remnants of the microsomal membranes.

MeSH Terms
Animals Male Membrane Proteins/analysis,metabolism Microsomes, Liver/analysis,drug effects,metabolism Molecular Weight Peptides/analysis Protein Binding Rats Ribosomes/metabolism Subcellular Fractions Surface-Active Agents/pharmacology
Chemicals
Membrane Proteins Peptides Surface-Active Agents
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kreibich G
Ulrich B L
Sabatini D D
References (73)
73 references, click to expand
  1. Patterns of organization of actin and myosin in normal and transformed cultured cells.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):994-8 PMID: 165499
  2. Enzyme profiles of mammalian bile.
    Biochim Biophys Acta. 1975 Apr 21;389(1):47-50 PMID: 166672
  3. Structural aspects of the membrane of the endoplasmic reticulum.
    Biochim Biophys Acta. 1975 Dec 29;415(4):411-72 PMID: 173395
  4. Proteins of rough microsomal membranes related to ribosome binding. II. Cross-linking of bound ribosomes to specific membrane proteins exposed at the binding sites.
    J Cell Biol. 1978 May;77(2):488-506 PMID: 418074
  5. Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.
    J Cell Biol. 1975 Dec;67(3):835-51 PMID: 811671
  6. On the involvement of cytochrome P-450 in the binding of ribosomes to a subfraction of rat-liver rapidly sedimenting endoplasmic reticulum.
    Eur J Biochem. 1977 Feb;72(3):595-603 PMID: 837930
  7. Isolation and characterization of membrane proteins responsible for attachment of polyribosomes to rough microsomal fraction of rat liver.
    Biochem J. 1977 Apr 15;164(1):53-66 PMID: 880237
  8. Accessibility of proteins in rat liver-free and membrane-bound ribosomes to lactoperoxidase-catalyzed iodination.
    J Biol Chem. 1977 Aug 10;252(15):5547-55 PMID: 885866
  9. Release of poly A(+) messenger RNA from rat liver rough microsomes upon disassembly of bound polysomes.
    J Cell Biol. 1977 Aug;74(2):414-27 PMID: 885910
  10. Nuclear ghosts: a nonmembranous structural component of mammalian cell nuclei.
    Science. 1976 Jul 30;193(4251):399-401 PMID: 935874
  11. Experimental disintegration of the nuclear envelope. Evidence for pore-connecting fibrils.
    J Cell Biol. 1976 Apr;69(1):1-18 PMID: 943400
  12. Isolation of nuclear pore complexes in association with a lamina.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1007-11 PMID: 1055359
  13. Tropomyosin antibody: the specific localization of tropomyosin in nonmuscle cells.
    J Cell Biol. 1975 Jun;65(3):549-61 PMID: 1094020
  14. Intracellular aspects of the process of protein synthesis.
    Science. 1975 Aug 1;189(4200):347-58 PMID: 1096303
  15. The isolation and characterization of nuclear ghosts from cultured HeLa cells.
    Biochemistry. 1975 Jul;14(13):3005-13 PMID: 1096936
  16. Actin in erythrocyte ghosts and its association with spectrin. Evidence for a nonfilamentous form of these two molecules in situ.
    J Cell Biol. 1975 Sep;66(3):508-20 PMID: 1099105
  17. Structural and functional aspects of the protein synthesizing apparatus in the rough endoplasmic reticulum.
    Adv Exp Med Biol. 1975;62:151-80 PMID: 1106131
  18. The role of membrane proteins and phospholipids in the interaction of ribosomes with endoplasmic reticulum membranes.
    Can J Biochem. 1975 Sep;53(9):1039-45 PMID: 1182590
  19. Characterization of the membrane matrix derived from the microsomal fraction of rat hepatocytes.
    Biochim Biophys Acta. 1976 Jun 17;436(2):387-98 PMID: 1276222
  20. Analytical study of microsomes and isolated subcellular membranes from rat liver. II. Preparation and composition of the microsomal fraction.
    J Cell Biol. 1974 Apr;61(1):201-12 PMID: 4150489
  21. Analytical study of microsomes and isolated subcellular membranes from rat liver. 3. Subfractionation of the microsomal fraction by isopycnic and differential centrifugation in density gradients.
    J Cell Biol. 1974 Apr;61(1):213-31 PMID: 4150490
  22. Selective release of content from microsomal vesicles without membrane disassembly. II. Electrophoretic and immunological characterization of microsomal subfractions.
    J Cell Biol. 1974 Jun;61(3):789-807 PMID: 4209578
  23. Identification of a nuclear protein matrix.
    Biochem Biophys Res Commun. 1974 Oct 23;60(4):1410-7 PMID: 4214419
  24. Actin and myosin and cell movement.
    CRC Crit Rev Biochem. 1974 Jan;2(1):1-65 PMID: 4273099
  25. Purification of hepatic microsomal membranes.
    Biochemistry. 1972 Aug 1;11(16):3128-35 PMID: 4339478
  26. The isolation of mouse hepatocyte gap junctions. Preliminary chemical characterization and x-ray diffraction.
    J Cell Biol. 1972 Sep;54(3):646-56 PMID: 4339819
  27. An improved cell fractionation procedure for the preparation of rat liver membrane-bound ribosomes.
    J Cell Biol. 1973 Jan;56(1):191-205 PMID: 4345164
  28. A comparative study of the molecular structures of the plasma membranes and the smooth and the rough endoplasmic-reticulum membranes from rat liver.
    Biochem J. 1972 Sep;129(3):781-8 PMID: 4349119
  29. A rapidly sedimenting fraction of rat liver endoplasmic reticulum.
    J Cell Sci. 1973 Sep;13(2):447-59 PMID: 4357366
  30. Microsomal membranes and the translational apparatus of eukaryotic cells.
    Fed Proc. 1973 Nov;32(11):2133-8 PMID: 4359600
  31. The synthesis and turnover of rat liver peroxisomes. I. Fractionation of peroxisome proteins.
    J Cell Biol. 1969 May;41(2):521-35 PMID: 4389026
  32. The effects of 3-methylcholanthrene and phenobarbital induction on the structure of the rat liver endoplasmic reticulum.
    Biochim Biophys Acta. 1974 Dec 10;373(2):197-210 PMID: 4429734
  33. Electrophoretic studies of the proteins of rat liver endoplasmic reticulum.
    Can J Biochem. 1974 Nov;52(11):1003-12 PMID: 4429864
  34. Ribosomal-membrane interaction: in vitro binding of ribosomes to microsomal membranes.
    J Mol Biol. 1974 Sep 25;88(3):559-80 PMID: 4449120
  35. A possible precursor of immunoglobulin light chains.
    Nat New Biol. 1972 Sep 27;239(91):117-20 PMID: 4507519
  36. The organization of proteins in the human red blood cell membrane. A review.
    J Cell Biol. 1974 Jul;62(1):1-19 PMID: 4600883
  37. Ribosome-membrane interaction. Nondestructive disassembly of rat liver rough microsomes into ribosomal and membranous components.
    J Cell Biol. 1973 Jan;56(1):206-29 PMID: 4682341
  38. Selective release of content from microsomal vesicles without membrane disassembly. I. Permeability changes induced by low detergent concentrations.
    J Cell Biol. 1973 Aug;58(2):436-62 PMID: 4729506
  39. Fractionation of membrane-bound polysomes, free polysomes, and nuclei from tissue-cultured cells.
    Eur J Biochem. 1973 Mar 1;33(2):368-73 PMID: 4735093
  40. The differentiation of rat liver endoplasmic reticulum membranes: apo--cytochrome P450 as a membrane protein.
    J Supramol Struct. 1973;1(6):471-89 PMID: 4782060
  41. Association of protein fractions and lipids from human erythrocyte membranes. II. Studies on a loosely bound protein fraction.
    Hoppe Seylers Z Physiol Chem. 1973 Jul;354(7):781-90 PMID: 4803510
  42. Selective solubilization of proteins from red blood cell membranes by protein perturbants.
    J Supramol Struct. 1973;1(3):220-32 PMID: 4804837
  43. Selective solubilization of proteins and phospholipids from red blood cell membranes by nonionic detergents.
    J Supramol Struct. 1973;1(3):233-48 PMID: 4804838
  44. Isopycnic centrifugation of free and membrane-bound polysomes from rat liver.
    Eur J Biochem. 1974 May 2;44(1):161-70 PMID: 4852603
  45. On the attachment of the nuclear pore complex.
    J Cell Biol. 1974 Sep;62(3):746-54 PMID: 4853439
  46. On the attachment of ribosomes to microsomal membranes.
    J Mol Biol. 1966 Aug;19(2):503-24 PMID: 4961331
  47. Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system.
    J Biol Chem. 1971 Oct 25;246(20):6328-34 PMID: 5127429
  48. Polysome interaction in vitro with microsomal membranes from rat liver.
    Biochem Biophys Res Commun. 1971 Dec 3;45(5):1212-8 PMID: 5135505
  49. Relation of ribonuclease and ribonuclease inhibitor to the isolation of polysomes from rat liver.
    Proc Natl Acad Sci U S A. 1966 May;55(5):1283-8 PMID: 5225526
  50. Multiple protein components of mammalian cell membranes.
    Proc Natl Acad Sci U S A. 1968 Dec;61(4):1370-7 PMID: 5249815
  51. Comparison of rat liver mitochondrial and microsomal membrane proteins.
    Proc Natl Acad Sci U S A. 1969 Jun;63(2):412-9 PMID: 5257131
  52. Peroxisomes (microbodies and related particles).
    Physiol Rev. 1966 Apr;46(2):323-57 PMID: 5325972
  53. Purification of urate oxidase; a sex dependent enzyme from rat liver.
    Arch Biochem Biophys. 1969 Dec;135(1):166-72 PMID: 5362920
  54. Gel electrophoresis patterns of the proteins of organelles isolated from bovine liver.
    Biochim Biophys Acta. 1970 Apr 21;203(2):283-90 PMID: 5441387
  55. Controlled proteolysis of nascent polypeptides in rat liver cell fractions. I. Location of the polypeptides within ribosomes.
    J Cell Biol. 1970 Apr;45(1):130-45 PMID: 5458992
  56. Controlled proteolysis of nascent polypeptides in rat liver cell fractions. II. Location of the polypeptides in rough microsomes.
    J Cell Biol. 1970 Apr;45(1):146-57 PMID: 5458993
  57. Size distribution of membrane proteins of rat liver and their relative rates of degradation.
    J Biol Chem. 1971 Apr 25;246(8):2574-83 PMID: 5553411
  58. The isolation of polysomes from rat liver. Contamination by membrane proteins with high affinity to ribosomes.
    Biochim Biophys Acta. 1971 Apr 8;232(4):705-16 PMID: 5556851
  59. Some features of the lipid composition of rat liver surface and cytoplasmic membranes.
    Chem Phys Lipids. 1968 Feb;2(1):144-6 PMID: 5698575
  60. Purification and properties of an enzyme from beef liver which catalyzes sulfhydryl-disulfide interchange in proteins.
    J Biol Chem. 1966 Apr 10;241(7):1562-7 PMID: 5946614
  61. Intracellular transport of secretory proteins in the pancreatic exocrine cell. I. Role of the peripheral elements of the Golgi complex.
    J Cell Biol. 1967 Aug;34(2):577-96 PMID: 6035647
  62. Electron microscopic examination of subcellular fractions. I. The preparation of representative samples from suspensions of particles.
    J Cell Biol. 1967 Jan;32(1):181-91 PMID: 10976209
  63. Liver microsomes; an integrated morphological and biochemical study.
    J Biophys Biochem Cytol. 1956 Mar 25;2(2):171-200 PMID: 13319380
  64. Pancreatic microsomes; an integrated morphological and biochemical study.
    J Biophys Biochem Cytol. 1956 Nov 25;2(6):671-90 PMID: 13398437
  65. A simple method for the isolation and purification of total lipides from animal tissues.
    J Biol Chem. 1957 May;226(1):497-509 PMID: 13428781
  66. The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid.
    J Biol Chem. 1960 Mar;235:769-75 PMID: 13793161
  67. The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation.
    Biochim Biophys Acta. 1962 May 14;55:571-83 PMID: 13893456
  68. URICASE: LOCALIZATION IN HEPATIC MICROBODIES.
    Science. 1964 Dec 4;146(3649):1316-8 PMID: 14207461
  69. THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. I. EVIDENCE FOR ITS HEMOPROTEIN NATURE.
    J Biol Chem. 1964 Jul;239:2370-8 PMID: 14209971
  70. THE MICROHETEROGENEITY OF PLASMA ALBUMINS. I. CRITICAL EVIDENCE FOR AND DESCRIPTION OF THE MICROHETEROGENEITY MODEL.
    J Biol Chem. 1965 Jun;240:2495-502 PMID: 14304859
  71. A small particulate component of the cytoplasm.
    J Biophys Biochem Cytol. 1955 Jan;1(1):59-68 PMID: 14381428
  72. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  73. The Fine Structure of the Parenchymal Cell of the Normal Rat Liver: I. General Observations.
    Am J Pathol. 1965 May;46(5):691-755 PMID: 19971027
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.77.2.464
Published
1978-05-00
Pages
464-87
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2110038
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.77.2.464 PMC Full Text View journal details
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