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PMID: 6502694 Published · ppublish English Journal Article

Volume regulation by Necturus gallbladder: basolateral KCl exit.

The Journal of membrane biology ·Vol. 81 ·No. 3 ·1984-00-00 ·Pages 219-32

Larson M, Spring KR

Abstract

Swelling of the epithelial cells of Necturus gallbladder caused by an 18% reduction in the osmolality of the mucosal bath is followed by rapid volume readjustment. This volume regulatory decrease requires Cl and is sensitive to the K and Cl gradients across the basolateral cell membrane. Volume regulatory decrease is not inhibited by amiloride, SITS, ouabain or bicarbonate removal. The process is blocked by bumetanide in the serosal bath. Measurement of the intracellular activities of K and Cl and the rate of volume regulation under five different experimental conditions showed that KCl exited from the cell across the basolateral membrane with a stoichiometry of 3 K to 2 Cl. This KCl exit process appears to be transiently activated following the reduction in osmolality of the mucosal perfusate.

MeSH Terms
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid/pharmacology Amiloride/pharmacology Animals Anions Biological Transport/drug effects Bumetanide/pharmacology Cell Membrane/drug effects,physiology Electric Conductivity/drug effects Epithelium/physiology Gallbladder/physiology Kinetics Necturus Osmolar Concentration Ouabain/pharmacology Potassium Chloride/metabolism
Chemicals
Anions Bumetanide 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid Ouabain Potassium Chloride Amiloride
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Larson M
Spring K R
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38 references, click to expand
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01868715
Published
1984-00-00
Pages
219-32
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01868715 View journal details
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