Home LiteratureArticle Details
PMID: 6517872 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The relationship of the rat brain 68 kDa microtubule-associated protein with synaptosomal plasma membranes and with the Drosophila 70 kDa heat-shock protein.

The Biochemical journal ·Vol. 224 ·No. 2 ·1984-12-01 ·Pages 677-80

Lim L, Hall C, Leung T, Whatley S

Abstract

A protein of molecular mass 68 kDa and pI5.6 is a major translation product of rat brain mRNA [Hall, Mahadevan, Whatley, Biswas & Lim (1984) Biochem. J. 219, 751-761]. In the rat brain this protein was associated with microtubule preparations and was present together with tubulin as a component of the synaptosomal plasma membranes, synaptic vesicles and post-synaptic structures. The brain mRNA for this protein was found to hybridize specifically to the Drosophila gene for the 70 kDa heat-shock protein, thus enabling its rapid isolation.

MeSH Terms
Animals Brain Chemistry Cell Membrane/metabolism Drosophila/genetics Electrophoresis, Polyacrylamide Gel Genes Heat-Shock Proteins/genetics Microtubule-Associated Proteins/analysis,metabolism Nucleic Acid Hybridization Protein Biosynthesis RNA, Messenger/genetics Rats Rats, Inbred Strains Synaptosomes/metabolism
Chemicals
Heat-Shock Proteins Microtubule-Associated Proteins RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lim L
Hall C
Leung T
Whatley S
References (23)
23 references, click to expand
  1. Adenine-rich polymer associated with rabbit reticulocyte messenger RNA.
    Nature. 1970 Aug 15;227(5259):710-2 PMID: 5432070
  2. The brain isoform of a key ATP-regulating enzyme, creatine kinase, is a phosphoprotein.
    Biochem J. 1984 Aug 15;222(1):139-44 PMID: 6477506
  3. Microtubule assembly in vitro. Purification of assembly-promoting factors.
    Eur J Biochem. 1977 Aug 15;78(1):167-74 PMID: 913395
  4. Synaptic proteins. Characterization of tubulin and actin and identification of a distinct postsynaptic density polypeptide.
    J Cell Biol. 1978 Oct;79(1):173-83 PMID: 568145
  5. The induction of gene activity in drosophilia by heat shock.
    Cell. 1979 Jun;17(2):241-54 PMID: 110462
  6. Presence of calmodulin in postsynaptic densities isolated from canine cerebral cortex.
    J Biol Chem. 1979 Sep 10;254(17):8690-6 PMID: 468848
  7. Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4927-31 PMID: 291909
  8. Calmodulin plays a pivotal role in cellular regulation.
    Science. 1980 Jan 4;207(4426):19-27 PMID: 6243188
  9. Fractionation of DNA fragments by polyethylene glycol induced precipitation.
    Methods Enzymol. 1980;65(1):347-53 PMID: 6246357
  10. The 68,000-dalton neurofilament-associated polypeptide is a component of nonneuronal cells and of skeletal myofibrils.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1541-5 PMID: 6990412
  11. Nucleotide sequences of heat shock activated genes in Drosophila melanogaster. I. Sequences in the regions of the 5' and 3' ends of the hsp 70 gene in the hybrid plasmid 56H8.
    Nucleic Acids Res. 1980 Jul 25;8(14):3105-23 PMID: 6255408
  12. Heat shock proteins are methylated in avian and mammalian cells.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3531-5 PMID: 6943552
  13. The characterization of tubulin in CNS membrane fractions.
    J Neurochem. 1981 Nov;37(5):1295-307 PMID: 7299399
  14. The polyadenylated RNA directing the synthesis of the rat myelin basic proteins is present in both free and membrane-bound forebrain polyribosomes.
    Biochem J. 1982 Feb 15;202(2):407-17 PMID: 6178399
  15. A regulatory upstream promoter element in the Drosophila hsp 70 heat-shock gene.
    Cell. 1982 Sep;30(2):517-28 PMID: 6814763
  16. Ca2+-dependent high-affinity complex formation between calmodulin and melittin.
    Biochem J. 1983 Jan 1;209(1):269-72 PMID: 6847615
  17. Expression of the major neurofilament subunit in chicken erythrocytes.
    Science. 1983 Aug 5;221(4610):553-6 PMID: 6346488
  18. Genetic regulation during heat shock and function of heat-shock proteins: a review.
    Can J Biochem Cell Biol. 1983 Jun;61(6):387-94 PMID: 6349753
  19. Neurone-specific enolase and creatine phosphokinase are protein components of rat brain synaptic plasma membranes.
    J Neurochem. 1983 Oct;41(4):1177-82 PMID: 6619855
  20. Heat shock proteins, first major products of zygotic gene activity in mouse embryo.
    Nature. 1983 Sep 22-28;305(5932):331-3 PMID: 6684733
  21. Characterization of translation products of the polyadenylated RNA of free and membrane-bound polyribosomes of rat forebrain.
    Biochem J. 1984 May 1;219(3):751-61 PMID: 6204641
  22. Alterations in the relative amounts of specific mRNA species in the developing human brain in Down's syndrome.
    Biochem J. 1984 May 15;220(1):179-87 PMID: 6204644
  23. Decrease in levels and rates of synthesis of tubulin and actin in developing rat brain.
    Brain Res. 1977 Feb;121(2):327-42 PMID: 832166
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2240677
Published
1984-12-01
Pages
677-80
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1144481
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2240677 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2