Home LiteratureArticle Details
PMID: 6524984 Published · ppublish English Journal Article

Characterisation of centromere (kinetochore) antigen reactive with sera of patients with a scleroderma variant (CREST syndrome).

Annals of the rheumatic diseases ·Vol. 43 ·No. 6 ·1984-12-00 ·Pages 819-24

Nishikai M, Okano Y, Yamashita H, Watanabe M

Abstract

Anticentromere (kinetochore) antibody is the marker antibody in CREST syndrome, but the precise molecular composition of the partner antigen has been poorly defined. This report describes for the first time a procedure for the successful extraction and biochemical characterisation of the centromere antigen molecule. The centromere antigen was extracted with 4M NaCl solution. The molecular weight of the partner antigen of the centromere antibody was determined to be 70 000 daltons by the SDS-PAGE and immunoblotting methods. A Sephacryl S-300 column experiment confirmed these results. Centromere antigenic activity was preserved at pHs between 3 and 11 and was resistant to three enzymes, trypsin, RNase, and DNase.

MeSH Terms
Adult Aged Antigens/analysis,isolation & purification Autoantibodies/immunology Calcinosis/immunology Centromere/immunology Chromatography, Gel Electrophoresis, Polyacrylamide Gel Esophageal Diseases/immunology Female Humans Male Middle Aged Molecular Weight Raynaud Disease/immunology Scleroderma, Systemic/immunology Species Specificity Syndrome Telangiectasis/immunology
Chemicals
Antigens Autoantibodies
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nishikai M
Okano Y
Yamashita H
Watanabe M
References (23)
23 references, click to expand
  1. Characteristics of a soluble nuclear antigen precipitating with sera of patients with systemic lupus erythematosus.
    J Immunol. 1966 Mar;96(3):464-71 PMID: 5932578
  2. Characterization of a soluble cytoplasmic antigen reactive with sera from patients with systemic lupus erythmatosus.
    J Immunol. 1969 Jan;102(1):117-22 PMID: 4179557
  3. Characterization of a soluble nuclear ribonucleoprotein antigen reactive with SLE sera.
    J Immunol. 1971 Nov;107(5):1281-90 PMID: 4330162
  4. Heterogeneity of RNA protein antigens reactive with sera of patients with systemic lupus erythematosus. Description of a cytoplasmic nonribosomal antigen.
    Arthritis Rheum. 1974 Jul-Aug;17(4):421-9 PMID: 4212051
  5. Antibodies to cellular antigens in Sjögren's syndrome.
    J Clin Invest. 1975 May;55(5):1067-73 PMID: 804494
  6. Antinuclear antibody with distinct specificity for polymyositis.
    J Clin Invest. 1977 Jan;59(1):176-8 PMID: 318657
  7. Identification and characterization of two new soluble nuclear antigens reactive with sera of patients with connective tissue diseases.
    Arthritis Rheum. 1978 Sep-Oct;21(7):803-10 PMID: 358992
  8. Antibodies reacting with ribosomal ribonucleoprotein in connective tissue diseases.
    Arthritis Rheum. 1979 Jan;22(1):87-93 PMID: 103560
  9. Characterization of a distinct nuclear acidic protein antigen (MA) and clinical findings in systemic lupus erythematosus patients with MA antibodies.
    J Clin Invest. 1979 Sep;64(3):820-3 PMID: 89119
  10. Identification of a nuclear protein (Scl-70) as a unique target of human antinuclear antibodies in scleroderma.
    J Biol Chem. 1979 Oct 25;254(20):10514-22 PMID: 385602
  11. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  12. Autoantibody to centromere (kinetochore) in scleroderma sera.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1627-31 PMID: 6966403
  13. The CREST syndrome: a distinct serologic entity with anticentromere antibodies.
    Am J Med. 1980 Oct;69(4):520-6 PMID: 6968511
  14. Purification and characterization of a nuclear non-histone basic protein (Mi-1) which reacts with anti-immunoglobulin sera and the sera of patients with dermatomyositis.
    Mol Immunol. 1980 Sep;17(9):1129-41 PMID: 6777667
  15. Distribution of kinetochore (centromere) antigen in mammalian cell nuclei.
    J Cell Biol. 1981 Jul;90(1):254-9 PMID: 7019222
  16. Characterization of a high molecular weight acidic nuclear protein recognized by autoantibodies in sera from patients with polymyositis-scleroderma overlap.
    J Clin Invest. 1981 Sep;68(3):611-20 PMID: 7276162
  17. Precipitating antibody to a soluble nuclear antigen "Ki" with specificity for systemic lupus erythematosus.
    Ryumachi. 1981;21 Suppl:129-40 PMID: 6979801
  18. Antinuclear antibodies in patients with Raynaud's phenomenon: clinical significance of anticentromere antibodies.
    Ann Rheum Dis. 1982 Aug;41(4):382-7 PMID: 7051989
  19. The 1982 revised criteria for the classification of systemic lupus erythematosus.
    Arthritis Rheum. 1982 Nov;25(11):1271-7 PMID: 7138600
  20. Anticentromere antibody. Clinical Correlations and association with favorable prognosis in patients with scleroderma variants.
    Arthritis Rheum. 1983 Jan;26(1):1-7 PMID: 6337593
  21. Autoimmune response directed against conserved determinants of nuclear envelope proteins in a patient with linear scleroderma.
    Proc Natl Acad Sci U S A. 1983 Jul;80(14):4374-8 PMID: 6192431
  22. Antibodies against distinct nuclear matrix proteins are characteristic for mixed connective tissue disease.
    Clin Exp Immunol. 1983 Oct;54(1):265-76 PMID: 6352106
  23. 1958 Revision of diagnostic criteria for rheumatoid arthritis.
    Bull Rheum Dis. 1958 Dec;9(4):175-6 PMID: 13596783
Article Info
Journal
Annals of the rheumatic diseases
Abbr.
Ann Rheum Dis
ISSN
0003-4967
DOI
10.1136/ard.43.6.819
Published
1984-12-00
Pages
819-24
Language
English
Region
England
NLM ID
0372355
PMCID
PMC1001545
Subset
IM
External Links
PubMed source DOI: 10.1136/ard.43.6.819 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2