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PMID: 6525626 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Flow cytometric analysis of mouse hepatocyte ploidy. II. The development of polyploidy pattern in four mice strains with different life spans.

Cell and tissue research ·Vol. 238 ·No. 3 ·1984-00-00 ·Pages 649-52

Severin E, Willers R, Bettecken T

Abstract

The development of liver ploidy in mice aged up to 24 months was investigated by flow cytometry in four mouse strains. A mathematical procedure was applied for correction of flow cytometry histograms. In two of the mouse strains, C3H and DBA, both cellular and nuclear ploidy proceed in the same way. The octoploid cell with two tetraploid nuclei is the most numerous cell type in adulthood. On the other hand, strain NZB and the out-bred strain NMRI show at the corresponding age a higher proportion of diploid cells with strikingly low proportions of 4c cells. In addition, high values of 16c cells and nuclei are present in NMRI. In all strains the proportion of binucleate hepatocytes is in the same range (60%). However, the strains differ in ploidy classes of binucleate cells. Development of liver polyploidization does not depend on life span of the specific strain.

MeSH Terms
Aging Animals Cell Nucleus/ultrastructure Flow Cytometry/methods Life Expectancy Liver/cytology,growth & development Mice Mice, Inbred C3H Mice, Inbred DBA Mice, Inbred Strains Ploidies Species Specificity
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Severin E
Willers R
Bettecken T
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20 references, click to expand
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Article Info
Journal
Cell and tissue research
Abbr.
Cell Tissue Res
ISSN
0302-766X
DOI
10.1007/BF00219884
Published
1984-00-00
Pages
649-52
Language
English
Region
Germany
NLM ID
0417625
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00219884 View journal details
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