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PMID: 6534032 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Measles virus gene expression in subacute sclerosing panencephalitis.

Virus research ·Vol. 1 ·No. 7 ·1984-10-00 ·Pages 585-95

Baczko K, Carter MJ, Billeter M, ter Meulen V

Abstract

RNA was extracted from the diseased brain of a case of human subacute sclerosing panencephalitis (SSPE) and analysed for the expression of measles-specific RNA. Measles virus-specific mRNAs were present, but the amount of matrix (M) protein mRNA was greatly reduced in comparison to lytically infected cells and phospho- (P) protein mRNA was hardly detectable whereas the level of the corresponding intermediate-sized (is-) RNA was greatly increased. RNA obtained from the human brain was also translated in vitro and measles virus nucleocapsid and P protein was produced. However, in marked contrast to control reactions M protein was not detected in the products formed by translation in vitro. These results indicate an impaired measles virus M protein mRNA synthesis in infected brain tissue.

MeSH Terms
Adolescent Antibodies, Viral/analysis Brain Chemistry Humans Male Measles virus/genetics,isolation & purification,physiology Protein Biosynthesis RNA, Messenger/analysis RNA, Viral/analysis Subacute Sclerosing Panencephalitis/microbiology Viral Matrix Proteins Viral Proteins/biosynthesis Virus Cultivation Virus Replication
Chemicals
Antibodies, Viral RNA, Messenger RNA, Viral Viral Matrix Proteins Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Baczko K
Carter M J
Billeter M
ter Meulen V
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28 references, click to expand
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Article Info
Journal
Virus research
Abbr.
Virus Res
ISSN
0168-1702
DOI
10.1016/0168-1702(84)90015-7
Published
1984-10-00
Pages
585-95
Language
English
Region
Netherlands
NLM ID
8410979
PMCID
PMC7134159
Subset
IM
External Links
PubMed source DOI: 10.1016/0168-1702(84)90015-7 PMC Full Text View journal details
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