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PMID: 6546388 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Light-regulated gene expression during maize leaf development.

The Journal of cell biology ·Vol. 98 ·No. 2 ·1984-02-00 ·Pages 558-64

Nelson T, Harpster MH, Mayfield SP, Taylor WC

Abstract

We have established schedules of expression during maize leaf development in light and darkness for the messenger RNAs (mRNAs) and polypeptides for ribulose 1,5-bisphosphate carboxylase (RuBPCase) subunits, phosphoenolpyruvate carboxylase (PEPCase), and the light-harvesting chlorophyll a/b-binding protein (LHCP). Levels of mRNAs were measured by hybridization with cloned probes, and proteins were measured by immunodetection on protein gel blots. The initial synthesis in leaves of all four mRNAs follows a light-independent schedule; illumination influences only the level to which each mRNA accumulates. The synthesis of RuBPCase small and large subunits and of PEPCase polypeptides also follows a light-independent schedule which is modified quantitatively by light. However, the accumulation of LHCP polypeptides absolutely requires illumination. The accumulation of each protein closely follows the accumulation of its mRNA during growth in light. Higher ratios of PEPCase and RuBPCase protein to mRNA occur during dark growth.

MeSH Terms
Cloning, Molecular DNA/isolation & purification Darkness Genes Light Phosphoenolpyruvate Carboxylase/genetics Plant Development Plant Proteins/genetics Plants/genetics RNA, Messenger/genetics Ribulose-Bisphosphate Carboxylase/genetics Zea mays/genetics,growth & development
Chemicals
Plant Proteins RNA, Messenger DNA Phosphoenolpyruvate Carboxylase Ribulose-Bisphosphate Carboxylase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nelson T
Harpster M H
Mayfield S P
Taylor W C
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36 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.98.2.558
Published
1984-02-00
Pages
558-64
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113091
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.98.2.558 PMC Full Text View journal details
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