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PMID: 6546586 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The effect of components of rabbit pulmonary surfactant on the activity of phospholipases.

The Journal of physiology ·Vol. 346 ·1984-01-00 ·Pages 439-48

Heath MF, Jacobson W

Abstract

This study investigates the ability of two components of pulmonary surfactant, protein and phosphatidylglycerol, to inhibit the action of phospholipases against phosphatidylcholine. Broncho-alveolar protein, prepared by de-lipidation of rabbit lung lavage material had an inhibitory effect on phospholipases A1 and A2 from rabbit lung lysosomes, comparable to the effect of bovine serum albumin. The degree of inhibition was found to increase with increasing enzyme activity. De-lipidated broncho-alveolar protein was separated into two fractions by gel chromatography. Inhibitory activity was associated only with the second peak, corresponding to rabbit albumin. The effect of phosphatidylglycerol (PG) on the activity of phospholipases A against dipalmitoyl phosphatidylcholine and unsaturated phosphatidylcholine (USPC) was investigated, and compared with the effects of two substrate analogues on the hydrolysis of USPC. There was no indication of true inhibition by PG, but some stimulation of USPC hydrolysis by 10 mol % PG. The relevance of these findings to the fate of surfactant in vivo is discussed.

MeSH Terms
Animals Chromatography, Gel Hydrolysis Lung/enzymology Lysosomes/enzymology Phosphatidylcholines/metabolism Phosphatidylglycerols/pharmacology Phospholipases/antagonists & inhibitors Phospholipases A/antagonists & inhibitors Proteolipids/pharmacology Pulmonary Surfactant-Associated Proteins Pulmonary Surfactants/pharmacology Rabbits Serum Albumin, Bovine/pharmacology
Chemicals
Phosphatidylcholines Phosphatidylglycerols Proteolipids Pulmonary Surfactant-Associated Proteins Pulmonary Surfactants Serum Albumin, Bovine Phospholipases Phospholipases A
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Heath M F
Jacobson W
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1984.sp015033
Published
1984-01-00
Pages
439-48
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1199510
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1984.sp015033 PMC Full Text View journal details
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