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PMID: 6547838 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Implication of a peroxisomal enzyme in the catabolism of glutaryl-CoA.

The Biochemical journal ·Vol. 221 ·No. 1 ·1984-07-01 ·Pages 203-11

Vamecq J, Van Hoof F

Abstract

A linear rate of H2O2 production is found when glutaryl-CoA is incubated with liver homogenates. We term this enzyme activity glutaryl-CoA oxidase. Its main characteristics are described and compared with those of glutaryl-CoA dehydrogenase (EC 1.3.99.7) and palmitoyl-CoA oxidase (EC 1.1.3.-). The latter enzyme catalyses the first step of peroxisomal beta-oxidation. Glutaryl-CoA oxidase shares several properties with palmitoyl-CoA oxidase. The activities of both enzymes in mouse liver are increased by feeding the animals with a clofibrate-containing diet. Subcellular fractionation of the liver homogenates on a linear sucrose gradient indicates that glutaryl-CoA oxidase is a peroxisomal enzyme.

MeSH Terms
Acyl Coenzyme A/metabolism Animals Centrifugation, Isopycnic Clofibrate/pharmacology Enzyme Activation/drug effects Glutaryl-CoA Dehydrogenase In Vitro Techniques Kinetics Liver/drug effects,enzymology,ultrastructure Male Microbodies/drug effects,enzymology Oxidoreductases/metabolism Oxidoreductases Acting on CH-CH Group Donors Rats Sulfates/pharmacology Zinc/pharmacology Zinc Sulfate
Chemicals
Acyl Coenzyme A Sulfates glutaryl-coenzyme A Zinc Sulfate Oxidoreductases Oxidoreductases Acting on CH-CH Group Donors glutaryl coenzyme A oxidase palmitoyl CoA oxidase Glutaryl-CoA Dehydrogenase Clofibrate Zinc
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Vamecq J
Van Hoof F
References (26)
26 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2210203
Published
1984-07-01
Pages
203-11
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1144021
Subset
IM
Grants
NIADDK NIH HHS · AM 9235 · United States
External Links
PubMed source DOI: 10.1042/bj2210203 PMC Full Text View journal details
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