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PMID: 6548015 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Subfamilies of the mouse major urinary protein (MUP) multi-gene family: sequence analysis of cDNA clones and differential regulation in the liver.

Nucleic acids research ·Vol. 12 ·No. 15 ·1984-08-10 ·Pages 6073-90

Kuhn NJ, Woodworth-Gutai M, Gross KW, Held WA

Abstract

The mouse major urinary proteins (MUPs) are the products of a multi-gene family of 30-35 genes whose members exhibit diverse tissue specific, developmental, and hormonal controls. Three cDNA clones corresponding to liver MUP mRNAs have been sequenced. Two of the clones (p499, C57BL/6 and p1057, BALB/c) share strong homology whereas a third clone (p199, C57BL/6) has diverged considerably from the others at the nucleic acid (85% homology) and protein (68% homology) levels. The 5' regions of p499 and p199 which show the most sequence divergence were subcloned and shown to hybridize to different liver MUP mRNAs. The p499-5' sequence was expressed in all MUP expressing tissues (liver, lachrymal, submaxillary and mammary) whereas the p199-5' sequence was expressed primarily in the liver and lachrymal. Analysis of liver RNA from mice in different endocrine states indicates that the p499-5' sequence is strongly regulated by thyroxine administration whereas the p199-5' sequence is not. Both sequences appear to be regulated by growth hormone and testosterone. Southern blot analysis of mouse genomic DNA indicates that there are multiple genes homologous to each sequence.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA/genetics Gene Expression Regulation Genes Growth Hormone/physiology Liver/physiology Mice Proteins/genetics Testosterone/physiology Tissue Distribution
Chemicals
Proteins major urinary proteins Testosterone Growth Hormone DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kuhn N J
Woodworth-Gutai M
Gross K W
Held W A
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25 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.15.6073
Published
1984-08-10
Pages
6073-90
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320058
Subset
IM
Grants
NCI NIH HHS · CA00449 · United States
NIGMS NIH HHS · GM24771 · United States
NIGMS NIH HHS · GM25023 · United States
Databases
GENBANK
X00907, X00908, X00909
External Links
PubMed source DOI: 10.1093/nar/12.15.6073 PMC Full Text View journal details
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