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PMID: 6548340 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An internal standard for porphyrin analysis.

Analytical biochemistry ·Vol. 140 ·No. 2 ·1984-08-01 ·Pages 360-5

Carlson RE, Sivasothy R, Dolphin D, Bernstein M, Shivji A

Abstract

The routine clinical analysis of the porphyrin precursors of heme requires an internal standard to determine the efficiency of the analytical procedure used. 2-Vinyl-4-hydroxymethyldeuteroporphyrin IX has been prepared as an internal standard. Its application to porphyrin analysis has been demonstrated using high-performance liquid chromatographic resolution of the uro- to protoporphyrins in normal and porphyric urine.

MeSH Terms

Chromatography, High Pressure Liquid Deuteroporphyrins/analysis,chemical synthesis Feces/analysis Humans Liver/analysis Porphyrins/analysis,chemical synthesis,standards,urine Protoporphyrins/analysis Reference Standards Uroporphyrins/analysis

Chemicals

Deuteroporphyrins Porphyrins Protoporphyrins Uroporphyrins 2-vinyl-4-(hydroxymethyl)deuteroporphyrin IX

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Carlson R E

Sivasothy R

Dolphin D

Bernstein M

Shivji A

Article Info

Journal

Analytical biochemistry

Abbr.

Anal Biochem

ISSN

0003-2697

DOI

10.1016/0003-2697(84)90178-7

Published

1984-08-01

Pages

360-5

Language

English

Region

United States

NLM ID

0370535

Subset

Grants

NIADDK NIH HHS · AM 17989 · United States

External Links

PubMed source DOI: 10.1016/0003-2697(84)90178-7 View journal details

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An internal standard for porphyrin analysis.

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